論文 - 詳細
| RRC ID | 19171 |
|---|---|
| 著者 | Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, Hatakeyama M, Handa H, Miyata A. |
| タイトル | Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide (PACAP) gene. |
| ジャーナル | Genes Cells |
| Abstract |
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide localized in the testis at concentration comparable to that found in the brain, suggesting involvement in spermatogenesis. In this study, we identified the human PACAP testis-specific exon (TSE) 10.9 kb upstream from the translational start site and found that the testis-specific transcript of the human PACAP gene was found to be spliced from the TSE into a region of intron 2 without a frameshift. The resulting PACAP precursor has no signal peptide, suggesting that PACAP functions physiologically in an intracrine manner in the testis. The 5'-flanking region of the TSE contains an 80-bp fragment with potent promoter activity in testicular F9 cell. Electrophoresis mobility shift assays showed that proteins from the F9 nuclear extract interacted specifically with the 80-bp fragment. DNA affinity chromatography allowed isolation of the specific proteins bound to the 80-bp fragment, two of which were identified as Poly (ADP-ribose) polymerase-1 (PARP-1) and TIA-1-related protein (TIAR) by mass spectrometry. By using their siRNAs, the depletion of their proteins in F9 cells affected the potent promoter activity of the 80-bp fragment, suggesting that they might be involved in the testis-specific gene expression of PACAP. |
| 巻・号 | 15(6) |
| ページ | 595-606 |
| 公開日 | 2010-6-1 |
| DOI | 10.1111/j.1365-2443.2010.01403.x |
| PII | GTC1403 |
| PMID | 20500521 |
| MeSH | Alternative Splicing Amino Acid Sequence Animals Base Sequence Binding Sites / genetics Cell Line, Tumor Electrophoretic Mobility Shift Assay Exons / genetics Gene Expression Profiling* Humans Luciferases / genetics Luciferases / metabolism Male Mice Molecular Sequence Data Pituitary Adenylate Cyclase-Activating Polypeptide / genetics* Pituitary Adenylate Cyclase-Activating Polypeptide / metabolism Poly(ADP-ribose) Polymerases / metabolism Promoter Regions, Genetic / genetics* Protein Binding RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Swiss 3T3 Cells Testis / metabolism* Transfection |
| IF | 1.655 |
| 引用数 | 7 |
| WOS 分野 | GENETICS & HEREDITY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NEC14(RCB0490) |