Reference - Detail
| RRC ID | 19177 |
|---|---|
| Author | Harada M, Kubo H, Abe T, Maezawa H, Otsuka H. |
| Title | Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique. |
| Journal | Jpn J Radiol |
| Abstract |
PURPOSE:The aim of this study was to select a suitable substrate candidate for dynamic nuclear polarization (DNP) studies and demonstrate its utility for evaluating intracellular metabolism. MATERIALS AND METHODS:Hyperpolarized substances included 1-(13)C-pyruvate (Pyr), 1-(13)C-glucose (Glc), and 1-(13)C-acetate. A DNP polarizer and a 600-MHz vertical small-bore scanner were used for (13)C-MR spectroscopic measurements. After polarization for 1 h, the dissolved solution was injected via a capillary line into the nuclear magnetic resonance tube in the scanner. The sequential spectra of the hyperpolarized (13)C-labeled substrates were acquired in durations of more than 120 s, and a thermal spectrum was obtained more than 1 h thereafter. FM3A cancer cells of mammary tumors were cultured for intracellular detection of the hyperpolarized (13)C-substances. RESULTS:The greatest sensitivity was found using Pyr with the longest T1 decay (51.5 s); and remarkably, the least sensitivity was observed using Glc with a signal decay of less than 2 s. An effective increase in sensitivity was shown using the other substances. The hyperpolarized intracellular study using (13)C-Pyr showed distinct elevation of lactate levels. CONCLUSION:The DNP technique is useful for evaluating intracellular metabolism. However, Glc is not suitable for use with the DNP technique. |
| Volume | 28(2) |
| Pages | 173-9 |
| Published | 2010-2-1 |
| DOI | 10.1007/s11604-009-0390-8 |
| PMID | 20182855 |
| MeSH | Acetates / metabolism* Animals Biomarkers, Tumor / metabolism Carbon Isotopes / metabolism* Cell Line, Tumor / metabolism Cells, Cultured Female Glucose / metabolism* Image Enhancement / methods Magnetic Resonance Spectroscopy / methods* Mammary Neoplasms, Experimental / metabolism* Mice Mice, Inbred C3H Pyruvic Acid / metabolism* Sensitivity and Specificity |
| IF | 1.439 |
| Times Cited | 13 |
| WOS Category | RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING |
| Resource | |
| Human and Animal Cells | FM3A(RCB0086) |