RRC ID |
1918
|
Author |
Hirano K, Miki Y, Hirai Y, Sato R, Itoh T, Hayashi A, Yamanaka M, Eda S, Beppu M.
|
Title |
A multifunctional shuttling protein nucleolin is a macrophage receptor for apoptotic cells.
|
Journal |
J Biol Chem
|
Abstract |
Early apoptotic Jurkat T cells undergo capping of CD43, and its polylactosaminyl saccharide chains serve as ligands for phagocytosis by macrophages. This suggests the presence of a polylactosaminoglycan-binding receptor on macrophages. Here we show that this receptor is nucleolin, a multifunctional shuttling protein present in nucleus, cytoplasm, and on the surface of some types of cells. Nucleolin was detected at the surface of macrophages, and anti-nucleolin antibody inhibited the binding of the early apoptotic cells to macrophages. Nucleolin-transfected HEK293 cells expressed nucleolin on the cell surface and bound the early apoptotic cells but not phosphatidylserine-exposing late apoptotic cells. This binding was inhibited by anti-nucleolin antibody, by polylactosamine-containing oligosaccharides, and by anti-CD43 antibody. Deletion of the antibody binding region of nucleolin resulted in loss of the apoptotic cell-binding ability. Moreover, truncated recombinant nucleolin in solution containing this region blocked the apoptotic cell binding to macrophages, and the blocking effect was cancelled by the oligosaccharides. These results indicate that nucleolin is a macrophage receptor for apoptotic cells.
|
Volume |
280(47)
|
Pages |
39284-93
|
Published |
2005-11-25
|
DOI |
10.1074/jbc.M505275200
|
PII |
S0021-9258(20)59137-0
|
PMID |
16135517
|
MeSH |
Amino Acid Sequence
Animals
Apoptosis / physiology*
Base Sequence
Cell Line
DNA, Complementary / genetics
Humans
In Vitro Techniques
Jurkat Cells
Macrophages / metabolism*
Male
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Molecular Weight
Monocytes / metabolism
Peptide Fragments / chemistry
Peptide Fragments / genetics
Peptide Fragments / metabolism
Phosphoproteins / chemistry
Phosphoproteins / genetics
Phosphoproteins / metabolism*
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Receptors, Cell Surface / chemistry
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Transfection
|
IF |
4.238
|
Times Cited |
49
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
Resource |
Human and Animal Cells |
Jurkat(RCB0806)
THP-1(RCB1189)
293(RCB1637) |