論文 - 詳細
| RRC ID | 19198 |
|---|---|
| 著者 | Fukushima T, Ohno J, Hayakawa T, Imayoshi R, Kawaguchi M, Doi Y, Kanaya K, Mitarai M. |
| タイトル | Polycationic protamine for water-insoluble complex formation with DNA. |
| ジャーナル | Dent Mater J |
| Abstract |
The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects. |
| 巻・号 | 29(5) |
| ページ | 529-35 |
| 公開日 | 2010-10-1 |
| DOI | 10.4012/dmj.2009-131 |
| PII | JST.JSTAGE/dmj/2009-131 |
| PMID | 20733264 |
| MeSH | 3T3 Cells Animals Anti-Bacterial Agents / pharmacology Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Biocompatible Materials / pharmacology Cell Survival / drug effects DNA / chemical synthesis* DNA / chemistry DNA / pharmacology DNA Adducts / chemical synthesis DNA Adducts / chemistry DNA Adducts / pharmacology Implants, Experimental Male Materials Testing Mice Microbial Sensitivity Tests Microscopy, Electron, Scanning Osteoblasts / drug effects Porosity Porphyromonas gingivalis / drug effects Prevotella intermedia / drug effects Protamines / chemical synthesis* Protamines / chemistry Protamines / pharmacology Rats Rats, Sprague-Dawley Salmon Solubility Staphylococcus aureus / drug effects Subcutaneous Tissue / drug effects Subcutaneous Tissue / pathology Surface Properties Water |
| IF | 1.359 |
| 引用数 | 17 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |