RRC ID 19198
著者 Fukushima T, Ohno J, Hayakawa T, Imayoshi R, Kawaguchi M, Doi Y, Kanaya K, Mitarai M.
タイトル Polycationic protamine for water-insoluble complex formation with DNA.
ジャーナル Dent Mater J
Abstract The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.
巻・号 29(5)
ページ 529-35
公開日 2010-10-1
DOI 10.4012/dmj.2009-131
PII JST.JSTAGE/dmj/2009-131
PMID 20733264
MeSH 3T3 Cells Animals Anti-Bacterial Agents / pharmacology Biocompatible Materials / chemical synthesis* Biocompatible Materials / chemistry Biocompatible Materials / pharmacology Cell Survival / drug effects DNA / chemical synthesis* DNA / chemistry DNA / pharmacology DNA Adducts / chemical synthesis DNA Adducts / chemistry DNA Adducts / pharmacology Implants, Experimental Male Materials Testing Mice Microbial Sensitivity Tests Microscopy, Electron, Scanning Osteoblasts / drug effects Porosity Porphyromonas gingivalis / drug effects Prevotella intermedia / drug effects Protamines / chemical synthesis* Protamines / chemistry Protamines / pharmacology Rats Rats, Sprague-Dawley Salmon Solubility Staphylococcus aureus / drug effects Subcutaneous Tissue / drug effects Subcutaneous Tissue / pathology Surface Properties Water
IF 1.359
引用数 17
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)