論文 - 詳細
| RRC ID | 19211 |
|---|---|
| 著者 | Kakinoki S, Yamaoka T. |
| タイトル | Stable modification of poly(lactic acid) surface with neurite outgrowth-promoting peptides via hydrophobic collagen-like sequence. |
| ジャーナル | Acta Biomater |
| Abstract |
Surface modification of poly(dl-lactic acid) (PLA) scaffolds has been performed using a biofunctional small peptide composed of collagen-like repetitive sequence and laminin-derived sequence (AG73-G(3)-(PPG)(5)) via hydrophobic interaction. The results of surface analysis suggest that AG73-G(3)-(PPG)(5) can be stably adsorbed onto PLA films via hydrophobic interaction at the (PPG)(5) region, and form an extracellular matrix-like layer composed of both structural and biosignalling sequences. In addition, neurite outgrowth of PC12 cells was observed on the AG73-G(3)-(PPG)(5)-adsorbed PLA film. These results indicate that AG73-G(3)-(PPG)(5) very effectively enhances neurite outgrowth activity on PLA films. The hydrophobic adsorption of collagen-like peptide bound to biosignalling molecules may be widely applied as a surface modifier of PLA films for tissue engineering. |
| 巻・号 | 6(6) |
| ページ | 1925-30 |
| 公開日 | 2010-6-1 |
| DOI | 10.1016/j.actbio.2009.12.001 |
| PII | S1742-7061(09)00531-5 |
| PMID | 19969110 |
| MeSH | Amino Acid Sequence Animals Biomimetic Materials / chemistry* Cell Culture Techniques / methods Cell Enlargement Cell Line Extracellular Matrix / chemistry Hydrophobic and Hydrophilic Interactions Lactic Acid / chemistry* Laminin / chemistry* Materials Testing Molecular Sequence Data Neurites / drug effects Neurites / physiology* Neurites / ultrastructure Neurons / cytology Neurons / physiology* Peptides / chemistry Polyesters Polymers / chemistry* Rats Surface Properties Tissue Engineering / methods* |
| IF | 7.242 |
| 引用数 | 12 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |