RRC ID 19212
Author Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M.
Title E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells.
Journal J. Biosci. Bioeng.
Abstract Conventionally, embryonic stem (ES) cells are cultured on a cell layer of mouse embryonic fibroblasts (MEFs) as feeder cells to support undifferentiated growth of ES cells. In this study, cell-cell interactions between mouse ES and feeder cells were artificially engineered via an epithelial cell adhesion molecule, E-cadherin, whose expression is considerable in ES cells. Mouse mesenchymal STO and NIH3T3 cells that were genetically engineered to express E-cadherin were used in ES cell cultures as feeder cells. ES cells cultured on the E-cadherin-expressing feeder cells maintained the expression of stem cell markers, alkaline phosphatase (AP), Oct3/4, Nanog and Sox2, and the efficiency of AP-positive colony formation was comparable to MEFs, and much better than parental STO and NIH3T3 cells. Furthermore, ES cells maintained on the E-cadherin-expressing feeder cells possessed the ability to differentiate into the three germ layers both in vitro and in vivo. The results indicated that E-cadherin expression in feeder cells could improve the performance of feeder cells, which may be further applicable to create new artificial feeder cell lines.
Volume 110(5)
Pages 582-7
Published 2010-11
DOI 10.1016/j.jbiosc.2010.06.002
PII S1389-1723(10)00192-1
PMID 20587371
MeSH Animals Base Sequence Cadherins / genetics* Cell Communication Cell Differentiation Cell Proliferation Coculture Techniques / methods* Colony-Forming Units Assay DNA Primers / genetics Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism* Gene Expression Genetic Engineering Mice NIH 3T3 Cells Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism Signal Transduction
IF 2.015
Times Cited 8
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Human and Animal Cells H-1 (AES0001)