論文 - 詳細
| RRC ID | 19212 |
|---|---|
| 著者 | Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M. |
| タイトル | E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Conventionally, embryonic stem (ES) cells are cultured on a cell layer of mouse embryonic fibroblasts (MEFs) as feeder cells to support undifferentiated growth of ES cells. In this study, cell-cell interactions between mouse ES and feeder cells were artificially engineered via an epithelial cell adhesion molecule, E-cadherin, whose expression is considerable in ES cells. Mouse mesenchymal STO and NIH3T3 cells that were genetically engineered to express E-cadherin were used in ES cell cultures as feeder cells. ES cells cultured on the E-cadherin-expressing feeder cells maintained the expression of stem cell markers, alkaline phosphatase (AP), Oct3/4, Nanog and Sox2, and the efficiency of AP-positive colony formation was comparable to MEFs, and much better than parental STO and NIH3T3 cells. Furthermore, ES cells maintained on the E-cadherin-expressing feeder cells possessed the ability to differentiate into the three germ layers both in vitro and in vivo. The results indicated that E-cadherin expression in feeder cells could improve the performance of feeder cells, which may be further applicable to create new artificial feeder cell lines. |
| 巻・号 | 110(5) |
| ページ | 582-7 |
| 公開日 | 2010-11-1 |
| DOI | 10.1016/j.jbiosc.2010.06.002 |
| PII | S1389-1723(10)00192-1 |
| PMID | 20587371 |
| MeSH | Animals Base Sequence Cadherins / genetics* Cell Communication Cell Differentiation Cell Proliferation Coculture Techniques / methods* Colony-Forming Units Assay DNA Primers / genetics Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism* Gene Expression Genetic Engineering Mice NIH 3T3 Cells Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism Signal Transduction |
| IF | 2.366 |
| 引用数 | 13 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | H-1(AES0001) |