論文 - 詳細
| RRC ID | 19213 |
|---|---|
| 著者 | Bando M, Hiroshima Y, Kataoka M, Herzberg MC, Ross KF, Shinohara Y, Yamamoto T, Nagata T, Kido J. |
| タイトル | Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1alpha. |
| ジャーナル | Immunol Cell Biol |
| Abstract |
Calprotectin is an antimicrobial complex composed of the S100A8 and S100A9 protein family subunits. Contributing to innate immunity, calprotectin expression is increased by interleukin-1alpha (IL-1alpha), which modulates keratinocyte differentiation. Keratinocyte growth factor (KGF) is produced by mesenchymal cells and has a mitogenic activity for epithelial cells. In this study, we investigated the effect of KGF on calprotectin expression in keratinocytes and modulation by IL-1alpha. Human keratinocytes were cultured with KGF in the presence or absence of a KGF receptor (KGFR) inhibitor or mitogen-activated protein kinase (MAPK) inhibitors. Calprotectin (S100A8/S100A9) expression was determined by northern blotting and enzyme-linked immunosorbent assay, respectively, whereas MAPK phosphorylation was analyzed by western blot analysis. KGF significantly decreased the expression of S100A8/S100A9-specific mRNAs and calprotectin protein. In the presence of KGF, KGFR inhibitor or extracellular-regulated kinase inhibitor restored KGF-downregulated expression of S100A8/S100A9. KGF increased IL-1alpha expression in keratinocytes, whereas IL-1alpha increased KGF expression in fibroblasts. Cocultured fibroblast and keratinocytes showed lower S100A8/S100A9 mRNA expression than keratinocytes alone in the presence or absence of IL-1alpha or KGF. These results suggest that fibroblast-derived KGF reduces or restricts calprotectin expression in keratinocytes, which supports our hypothesis that calprotectin expression in keratinocytes is modulated by factors associated with epithelial-mesenchymal interactions. |
| 巻・号 | 88(3) |
| ページ | 328-33 |
| 公開日 | 2010-1-1 |
| DOI | 10.1038/icb.2009.104 |
| PII | icb2009104 |
| PMID | 20065999 |
| MeSH | Calgranulin A / biosynthesis* Calgranulin B / biosynthesis* Cell Line Coculture Techniques Fibroblast Growth Factor 7 / metabolism* Fibroblast Growth Factor 7 / pharmacology Fibroblasts / metabolism Gene Expression Regulation / drug effects Gene Expression Regulation / physiology* Humans Interleukin-1alpha / metabolism* Interleukin-1alpha / pharmacology Keratinocytes / metabolism* Mitogen-Activated Protein Kinase Kinases / metabolism Phosphorylation / drug effects Phosphorylation / physiology |
| IF | 3.764 |
| 引用数 | 16 |
| WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |