RRC ID |
19226
|
著者 |
Ishii T, Yasuchika K, Suemori H, Nakatsuji N, Ikai I, Uemoto S.
|
タイトル |
Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma.
|
ジャーナル |
Cancer Lett
|
Abstract |
We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics.
|
巻・号 |
294(1)
|
ページ |
25-34
|
公開日 |
2010-8-1
|
DOI |
10.1016/j.canlet.2010.01.019
|
PII |
S0304-3835(10)00045-5
|
PMID |
20149523
|
MeSH |
Aged
Aged, 80 and over
Animals
Bile Duct Neoplasms / metabolism*
Bile Duct Neoplasms / pathology
Bile Ducts, Intrahepatic
Biomarkers / metabolism
Cell Division
Cell Line, Tumor
Cholangiocarcinoma / metabolism*
Cholangiocarcinoma / pathology
Enhancer Elements, Genetic
Female
Genes, Reporter
Genetic Vectors
Green Fluorescent Proteins / genetics
Humans
Male
Mice
Mice, Inbred NOD
Middle Aged
Neoplastic Stem Cells / metabolism*
Plasmids
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Transplantation, Heterologous
alpha-Fetoproteins / biosynthesis
alpha-Fetoproteins / genetics*
|
IF |
7.36
|
引用数 |
23
|
WOS 分野
|
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
SSP-25(RCB1293)
RBE(RCB1292) |