論文 - 詳細
| RRC ID | 19226 |
|---|---|
| 著者 | Ishii T, Yasuchika K, Suemori H, Nakatsuji N, Ikai I, Uemoto S. |
| タイトル | Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma. |
| ジャーナル | Cancer Lett |
| Abstract |
We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics. |
| 巻・号 | 294(1) |
| ページ | 25-34 |
| 公開日 | 2010-8-1 |
| DOI | 10.1016/j.canlet.2010.01.019 |
| PII | S0304-3835(10)00045-5 |
| PMID | 20149523 |
| MeSH | Aged Aged, 80 and over Animals Bile Duct Neoplasms / metabolism* Bile Duct Neoplasms / pathology Bile Ducts, Intrahepatic Biomarkers / metabolism Cell Division Cell Line, Tumor Cholangiocarcinoma / metabolism* Cholangiocarcinoma / pathology Enhancer Elements, Genetic Female Genes, Reporter Genetic Vectors Green Fluorescent Proteins / genetics Humans Male Mice Mice, Inbred NOD Middle Aged Neoplastic Stem Cells / metabolism* Plasmids Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction Transfection Transplantation, Heterologous alpha-Fetoproteins / biosynthesis alpha-Fetoproteins / genetics* |
| IF | 7.36 |
| 引用数 | 23 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | SSP-25(RCB1293) RBE(RCB1292) |