RRC ID 1924
Author Nishio K, Qiao S, Yamashita H.
Title Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1).
Journal J Mol Histol
Abstract The expression status of mitochondrial uncoupling protein 2 (UCP2) was investigated in undifferentiated mouse myeloid leukemia (M1) and its differentiated macrophage-like cells (Mm1). Mm1 cells have a high ability of phagocytosis along with significantly high levels of reactive oxygen species (ROS) production, UCP2 protein and manganese superoxide dismutase (Mn-SOD), in contrast to undifferentiated leukemia cells (M1). Mm1 cells expressed 10-fold more UCP2 protein compared with undifferentiated M1 cells, although the UCP2 mRNA levels in both cell types were similar. The higher expression of UCP2 in the Mm1 cells suggests a regulatory role of UCP2 in the ROS production. Furthermore, the transfection of UCP2-GFP-expression vector in Mm1 cells dissipated the mitochondrial membrane potential and reduced ROS production, which was shown by their direct visualization using MitoTracker Red CM-H2Xros. The macrophage gp91phox protein, a membrane catalytic component of the NADPH oxidase complex, was at a similar level in both of UCP2-GFP expressed and non-expressed Mm1 cells. These results suggest that the UCP2 protein of the undifferentiated cell is regulated at a quite low level and the higher UCP2 protein of the differentiated macrophages involves with the regulation of ROS production.
Volume 36(1-2)
Pages 35-44
Published 2005-2-1
DOI 10.1007/s10735-004-2915-x
PMID 15703997
MeSH Animals Cell Line Gene Expression Regulation Ion Channels Leukemia, Myeloid / metabolism Macrophages / enzymology Macrophages / metabolism* Membrane Glycoproteins / analysis Membrane Glycoproteins / metabolism Membrane Potentials Membrane Transport Proteins / analysis Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Mice Mitochondria / chemistry Mitochondria / metabolism Mitochondrial Proteins / analysis Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* NADPH Oxidase 2 NADPH Oxidases / analysis NADPH Oxidases / metabolism Phagocytosis RNA, Messenger / genetics RNA, Messenger / metabolism Rats Reactive Oxygen Species / metabolism* Stem Cells / metabolism* Superoxide Dismutase / analysis Superoxide Dismutase / metabolism Transcriptional Activation Uncoupling Protein 2
IF 2.937
Times Cited 22
Human and Animal Cells M1 Mm1