論文 - 詳細
RRC ID | 1924 |
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著者 | Nishio K, Qiao S, Yamashita H. |
タイトル | Characterization of the differential expression of uncoupling protein 2 and ROS production in differentiated mouse macrophage-cells (Mm1) and the progenitor cells (M1). |
ジャーナル | J Mol Histol |
Abstract |
The expression status of mitochondrial uncoupling protein 2 (UCP2) was investigated in undifferentiated mouse myeloid leukemia (M1) and its differentiated macrophage-like cells (Mm1). Mm1 cells have a high ability of phagocytosis along with significantly high levels of reactive oxygen species (ROS) production, UCP2 protein and manganese superoxide dismutase (Mn-SOD), in contrast to undifferentiated leukemia cells (M1). Mm1 cells expressed 10-fold more UCP2 protein compared with undifferentiated M1 cells, although the UCP2 mRNA levels in both cell types were similar. The higher expression of UCP2 in the Mm1 cells suggests a regulatory role of UCP2 in the ROS production. Furthermore, the transfection of UCP2-GFP-expression vector in Mm1 cells dissipated the mitochondrial membrane potential and reduced ROS production, which was shown by their direct visualization using MitoTracker Red CM-H2Xros. The macrophage gp91phox protein, a membrane catalytic component of the NADPH oxidase complex, was at a similar level in both of UCP2-GFP expressed and non-expressed Mm1 cells. These results suggest that the UCP2 protein of the undifferentiated cell is regulated at a quite low level and the higher UCP2 protein of the differentiated macrophages involves with the regulation of ROS production. |
巻・号 | 36(1-2) |
ページ | 35-44 |
公開日 | 2005-2-1 |
DOI | 10.1007/s10735-004-2915-x |
PMID | 15703997 |
MeSH | Animals Cell Line Gene Expression Regulation Ion Channels Leukemia, Myeloid / metabolism Macrophages / enzymology Macrophages / metabolism* Membrane Glycoproteins / analysis Membrane Glycoproteins / metabolism Membrane Potentials Membrane Transport Proteins / analysis Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Mice Mitochondria / chemistry Mitochondria / metabolism Mitochondrial Proteins / analysis Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism* NADPH Oxidase 2 NADPH Oxidases / analysis NADPH Oxidases / metabolism Phagocytosis RNA, Messenger / genetics RNA, Messenger / metabolism Rats Reactive Oxygen Species / metabolism* Stem Cells / metabolism* Superoxide Dismutase / analysis Superoxide Dismutase / metabolism Transcriptional Activation Uncoupling Protein 2 |
IF | 2.531 |
引用数 | 22 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | M1(RCB0169) Mm1(RCB0748) |