論文 - 詳細
| RRC ID | 19258 |
|---|---|
| 著者 | Sohn SH, Ko E, Oh BG, Kim J, Choi E, Kim SH, Kim Y, Shin M, Hong M, Bae H. |
| タイトル | Global gene analysis of Erigeron canadensis-treated TNF-alpha-, IL-4- and IL-1beta-stimulated A549 human epithelial cells. |
| ジャーナル | Ann Nutr Metab |
| Abstract |
BACKGROUND/AIMS:This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-alpha (TNF-alpha)-, interleukin (IL)-4- and IL-1beta-induced stimulation of A549 cells. METHODS:In the present study, the anti-inflammatory effects of EC on TNF-alpha-, IL-4- and IL-1beta-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis. RESULTS:Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1beta, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-alpha, IL-4 and IL-1beta. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells. CONCLUSIONS:EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC. |
| 巻・号 | 54(3) |
| ページ | 227-35 |
| 公開日 | 2009-1-1 |
| DOI | 10.1159/000225378 |
| PII | 000225378 |
| PMID | 19521071 |
| MeSH | Algorithms Analysis of Variance Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Cell Line, Tumor Chemokine CCL11 / metabolism Cytokines / immunology* Dietary Supplements Epithelial Cells / drug effects* Epithelial Cells / immunology Epithelial Cells / metabolism Erigeron* Gene Expression Regulation / drug effects* Humans Intercellular Adhesion Molecule-1 / genetics Intercellular Adhesion Molecule-1 / metabolism Interleukin-1beta / immunology Interleukin-4 / immunology Interleukin-8 / genetics Interleukin-8 / metabolism MAP Kinase Signaling System / drug effects Oligonucleotide Array Sequence Analysis Plant Extracts / pharmacology* Pulmonary Alveoli / cytology* RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha / immunology Vascular Cell Adhesion Molecule-1 / genetics Vascular Cell Adhesion Molecule-1 / metabolism |
| IF | 2.848 |
| 引用数 | 4 |
| WOS 分野 | NUTRITION & DIETETICS ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | EoL-1 cell(RCB0641) |