論文 - 詳細
| RRC ID | 19264 |
|---|---|
| 著者 | Itoh T, Nozawa Y, Akao Y. |
| タイトル | MicroRNA-141 and -200a are involved in bone morphogenetic protein-2-induced mouse pre-osteoblast differentiation by targeting distal-less homeobox 5. |
| ジャーナル | J Biol Chem |
| Abstract |
MicroRNAs (miRs) are endogenously expressed 18-25-nucleotide RNAs that regulate gene expression through translational repression by binding to a target mRNA. Recently, it was indicated that miRs act as key regulators in cell differentiation, cell growth, and cell death. In osteogenesis, several miRs (for example miR-26a, -125b, -133, and -135) regulate osteoblast cell growth or differentiation in human adipose tissue-derived stem cells, mouse mesenchymal ST2 stem cells, and mouse premyogenic C2C12 cells. Additionally, Smad proteins control Drosha-mediated miR maturation. Therefore, miRs are closely related to osteogenesis. Here we investigated miR expression profile by an miR array and identified the candidate miRs, miR-141 and -200a, as pre-osteoblast differentiation-related miRs. The effects of miR-141 and -200a on pre-osteoblast differentiation were examined by using transfection of murine pre-osteoblastic MC3T3-E1 cells with mature miR-141 or -200a and antisense inhibitor for miR-141 or -200a. It was shown that miR-141 and -200a remarkably modulated the BMP-2-induced pre-osteoblast differentiation through the translational repression of Dlx5, which is a bone-generating transcription factor expressed in pre-osteoblast differentiation. Furthermore, it was indicated that Dlx5 is a common target of miR-141 and -200a by using a luciferase reporter assay. Thus, we have observed for the first time that miR-141 and -200a are involved in pre-osteoblast differentiation in part by regulating the expression of Dlx5. |
| 巻・号 | 284(29) |
| ページ | 19272-9 |
| 公開日 | 2009-7-17 |
| DOI | 10.1074/jbc.M109.014001 |
| PII | S0021-9258(19)81134-1 |
| PMID | 19454767 |
| PMC | PMC2740552 |
| MeSH | Animals Base Sequence Blotting, Western Bone Morphogenetic Protein 2 / pharmacology* Cell Differentiation / drug effects* Cell Line Gene Expression Profiling Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Luciferases / genetics Luciferases / metabolism Mice MicroRNAs / genetics MicroRNAs / metabolism* Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Reverse Transcriptase Polymerase Chain Reaction Transfection |
| IF | 4.238 |
| 引用数 | 141 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |