RRC ID 19270
著者 Miki Y, Itoh T, Hirano K, Eda S, Hayashi A, Yamanaka M, Beppu M.
タイトル Clearance of oxidatively damaged cells by macrophages: recognition of glycoprotein clusters by macrophage-surface nucleolin as early apoptotic cells.
ジャーナル Biol Pharm Bull
Abstract The mechanism of macrophage recognition of oxidatively damaged cells was investigated. Jurkat T cells exposed to various concentrations of H(2)O(2) were bound and phagocytosed by macrophages. The cells exposed to 0.1 mM H(2)O(2) were best bound. The cell-surface ligands recognized by macrophages were suggested to be sialylpolylactosaminyl sugar chains of a major sialoglycoprotein CD43 because 1) the cell binding was inhibited by oligosaccharides containing sialylpolylactosaminyl chains, and their inhibitory activity was destroyed by a polylactosamine-cleaving enzyme endo-beta-galactosidase, and by neuraminidase; 2) the oxidized Jurkat cells pretreated with either glycosidase or with anti-CD43 antibody were not bound. The macrophage receptor involved in the binding was suggested to be cell-surface nucleolin because 1) anti-nucleolin antibody inhibited the binding; 2) nucleolin-transfected HEK293 cells bound the oxidized cells; and 3) this binding was inhibited by anti-nucleolin antibody and by anti-CD43 antibody. CD43 on oxidized Jurkat cells tended to form clusters in good accordance with their susceptibility to the macrophage binding. CD43 clustering and the oxidized-cell binding to macrophages were prevented by a caspase inhibitor Z-VAD-fmk, suggesting that the oxidized and bound cells were undergoing apoptosis. Indeed, caspase-3 activity of Jurkat cells increased by the oxidation. These results suggest that moderately oxidized cells undergo apoptosis and are recognized by macrophages as early apoptotic cells.
巻・号 32(4)
ページ 564-72
公開日 2009-4-1
DOI 10.1248/bpb.32.564
PII JST.JSTAGE/bpb/32.564
PMID 19336885
MeSH Apoptosis / physiology* Caspases / metabolism Cell Line Cell Nucleus / physiology Cell Nucleus / ultrastructure Cytosol / physiology Cytosol / ultrastructure Glycoproteins / chemistry* Humans Jurkat Cells Leukosialin / chemistry Macrophages / physiology* Membrane Proteins / chemistry Oxidation-Reduction Oxidative Stress / physiology* Phosphoproteins / chemistry* RNA-Binding Proteins / chemistry*
IF 1.863
引用数 8
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 THP-1(RCB1189) 293(RCB1637)