論文 - 詳細
| RRC ID | 19270 |
|---|---|
| 著者 | Miki Y, Itoh T, Hirano K, Eda S, Hayashi A, Yamanaka M, Beppu M. |
| タイトル | Clearance of oxidatively damaged cells by macrophages: recognition of glycoprotein clusters by macrophage-surface nucleolin as early apoptotic cells. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
The mechanism of macrophage recognition of oxidatively damaged cells was investigated. Jurkat T cells exposed to various concentrations of H(2)O(2) were bound and phagocytosed by macrophages. The cells exposed to 0.1 mM H(2)O(2) were best bound. The cell-surface ligands recognized by macrophages were suggested to be sialylpolylactosaminyl sugar chains of a major sialoglycoprotein CD43 because 1) the cell binding was inhibited by oligosaccharides containing sialylpolylactosaminyl chains, and their inhibitory activity was destroyed by a polylactosamine-cleaving enzyme endo-beta-galactosidase, and by neuraminidase; 2) the oxidized Jurkat cells pretreated with either glycosidase or with anti-CD43 antibody were not bound. The macrophage receptor involved in the binding was suggested to be cell-surface nucleolin because 1) anti-nucleolin antibody inhibited the binding; 2) nucleolin-transfected HEK293 cells bound the oxidized cells; and 3) this binding was inhibited by anti-nucleolin antibody and by anti-CD43 antibody. CD43 on oxidized Jurkat cells tended to form clusters in good accordance with their susceptibility to the macrophage binding. CD43 clustering and the oxidized-cell binding to macrophages were prevented by a caspase inhibitor Z-VAD-fmk, suggesting that the oxidized and bound cells were undergoing apoptosis. Indeed, caspase-3 activity of Jurkat cells increased by the oxidation. These results suggest that moderately oxidized cells undergo apoptosis and are recognized by macrophages as early apoptotic cells. |
| 巻・号 | 32(4) |
| ページ | 564-72 |
| 公開日 | 2009-4-1 |
| DOI | 10.1248/bpb.32.564 |
| PII | JST.JSTAGE/bpb/32.564 |
| PMID | 19336885 |
| MeSH | Apoptosis / physiology* Caspases / metabolism Cell Line Cell Nucleus / physiology Cell Nucleus / ultrastructure Cytosol / physiology Cytosol / ultrastructure Glycoproteins / chemistry* Humans Jurkat Cells Leukosialin / chemistry Macrophages / physiology* Membrane Proteins / chemistry Oxidation-Reduction Oxidative Stress / physiology* Phosphoproteins / chemistry* RNA-Binding Proteins / chemistry* |
| IF | 1.863 |
| 引用数 | 8 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) 293(RCB1637) |