RRC ID 19271
Author Yamasaki K, Hagiwara H.
Title Excess iron inhibits osteoblast metabolism.
Journal Toxicol. Lett.
Abstract Hemochromatosis is an iron overload disorder associated with osteopenia and osteoporosis. To learn more about the effects of iron on bone cells, we examined the effects of ferric ion on the proliferation, differentiation, and mineralization of two types of cultured osteoblasts, the cell line MC3T3-E1 and rat calvarial osteoblast-like (ROB) cells. We used ferric ammonium citrate (FAC) as a donor of ferric ion, and FAC inhibited the proliferation of MC3T3-E1 cells in a dose-dependent manner. FAC (0.1-1 microg/ml) inhibited indices of osteoblast differentiation, such as the expression of type I collagen (mRNA and protein), the activity of alkaline phosphatase, and the deposition of calcium by osteoblasts. These results suggest that iron overload might give rise to osteoporosis by inhibiting osteoblast proliferation and differentiation.
Volume 191(2-3)
Pages 211-5
Published 2009-12-15
DOI 10.1016/j.toxlet.2009.08.023
PII S0378-4274(09)01421-0
PMID 19735707
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Bone and Bones / cytology Bone and Bones / drug effects Bone and Bones / metabolism Calcification, Physiologic / drug effects Calcium / metabolism Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Cell Survival / drug effects Citric Acid / pharmacology Collagen Type I / metabolism Iron / toxicity* Mice Osteoblasts / drug effects Osteoblasts / metabolism* Quaternary Ammonium Compounds / pharmacology Rats Reverse Transcriptase Polymerase Chain Reaction Skull / drug effects Skull / metabolism
IF 3.166
Times Cited 61
WOS Category TOXICOLOGY
Resource
Human and Animal Cells MC3T3-E1 (RCB1126)