| RRC ID |
19279
|
| 著者 |
Zhu X, Hatanaka K, Yamagata T, Sato T.
|
| タイトル |
Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS.
|
| ジャーナル |
Electrophoresis
|
| Abstract |
A glycosphingolipid analogue (12-azidododecyl beta-lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE-ESI-MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12-azidododecyl beta-lactoside (Lac-C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac-C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CE-ESI-MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity.
|
| 巻・号 |
30(20)
|
| ページ |
3519-26
|
| 公開日 |
2009-10-1
|
| DOI |
10.1002/elps.200800719
|
| PMID |
19813238
|
| MeSH |
Animals
Azides / chemistry*
Cell Line, Tumor
Cells, Cultured
Electrophoresis, Capillary / methods*
Glycosphingolipids / biosynthesis
Glycosphingolipids / chemistry*
Glycosylation
Humans
Lactose / analogs & derivatives*
Lactose / chemistry
Oligosaccharides / chemical synthesis*
Spectrometry, Mass, Electrospray Ionization / methods*
|
| IF |
3.081
|
| 引用数 |
4
|
|
WOS 分野
|
CHEMISTRY, ANALYTICAL
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ヒト・動物細胞 |
MDCK(RCB0995) |
