論文 - 詳細
| RRC ID | 19287 |
|---|---|
| 著者 | Kinugawa M, Fukuzawa S, Tachibana K. |
| タイトル | Skeletal protein protection: the mode of action of an anti-osteoporotic marine alkaloid, norzoanthamine. |
| ジャーナル | J Bone Miner Metab |
| Abstract |
Bone is composed of mineralized collagen fibrils. A marine alkaloid, norzoanthamine, accelerates the formation of a collagen-hydroxyapatite composite and enhances collagen release from an immobilized matrix vesicle model. Norzoanthamine recognizes a peptide chain nonspecifically and stabilizes its secondary structure, and collagen has polyvalent binding sites for norzoanthamine. This collagen-norzoanthamine supramolecular association is considered to be one of the most significant modes of action for enhancement of bone formation. The facts that norzoanthamine is nontoxic and that it has a collagen protective activity indicate that it may provide significant therapeutic benefits. In particular, it may be a promising drug candidate for osteoporosis treatment and prevention. Interestingly, norzoanthamine suppressed the proteolysis of not only collagen but also elastin and bovine serum albumin, so it apparently has a universal protective effect of guarding extracellular matrix proteins from degradation. This result suggests that norzoanthamine protect skeletal proteins in the host animal body from external stresses and possibly enhance survival. |
| 巻・号 | 27(3) |
| ページ | 303-14 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/s00774-009-0049-7 |
| PMID | 19277454 |
| MeSH | Alkaline Phosphatase / metabolism Alkaloids / chemistry Alkaloids / pharmacology Alkaloids / therapeutic use Animals Azepines / chemistry Azepines / pharmacology* Azepines / therapeutic use* Bone and Bones / drug effects Bone and Bones / metabolism* Cell Shape / drug effects Cell Survival / drug effects Circular Dichroism Collagen / metabolism Durapatite / metabolism Fluorescein-5-isothiocyanate Heterocyclic Compounds, 4 or More Rings / chemistry Heterocyclic Compounds, 4 or More Rings / pharmacology Heterocyclic Compounds, 4 or More Rings / therapeutic use Matrix Metalloproteinase Inhibitors Mice Nephelometry and Turbidimetry Osteoporosis / drug therapy* Pancreatic Elastase / antagonists & inhibitors Protease Inhibitors / pharmacology Protein Processing, Post-Translational / drug effects Proteins / metabolism* Quinolines / chemistry Quinolines / pharmacology* Quinolines / therapeutic use* Spectrophotometry, Ultraviolet Trypsin / metabolism |
| IF | 2.297 |
| 引用数 | 18 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |