Reference - Detail
RRC ID | 19313 |
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Author | Munekata K, Sakamoto K. |
Title | Forkhead transcription factor Foxo1 is essential for adipocyte differentiation. |
Journal | In Vitro Cell Dev Biol Anim |
Abstract |
We analyzed the physiological role of forkhead box class O 1 (Foxo1) in adipocyte differentiation by suppressing Foxo1 mRNAwith siRNA specific for Foxo1.Mouse 3T3-L1 preadipocytes infected with an adenovirus expressing Foxo1-siRNA showed a marked decrease in lipid droplet formation when induced to differentiate into adipocytes. Adipocyte differentiation was most severely inhibited by exposing cells to Foxo1-siRNA before induction of differentiation. The incorporation of fluorescent-labeled glucose and fatty acid was significantly inhibited in cells deficient in Foxo1. RTPCR revealed that downregulation of Foxo1 decreased the expression of the transcription factors, PPAR-γ and C/EBP-α. By comparison, Foxo1-siRNA did not affect the expression of C/EBP-β or C/EBP-δ during the early period of adipocyte differentiation. These results indicate that Foxo1 plays an essential role in adipocyte differentiation, especially at the very early stage of terminal adipocyte differentiation. |
Volume | 45(10) |
Pages | 642-51 |
Published | 2009-12-1 |
DOI | 10.1007/s11626-009-9230-5 |
PMID | 19585174 |
MeSH | 3T3 Cells Adipocytes / cytology* Adipocytes / metabolism Adipogenesis / genetics Animals COS Cells Cell Differentiation / genetics* Chlorocebus aethiops Forkhead Box Protein O1 Forkhead Transcription Factors / antagonists & inhibitors Forkhead Transcription Factors / physiology* Lipid Metabolism / genetics Mice RNA Interference RNA, Messenger / metabolism |
IF | 1.665 |
Times Cited | 45 |
WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
Resource | |
Human and Animal Cells | 293(RCB1637) COS-7(RCB0539) |