論文 - 詳細
| RRC ID | 1933 |
|---|---|
| 著者 | Watanabe D, Ezoe S, Fujimoto M, Kimura A, Saito Y, Nagai H, Tachibana I, Matsumura I, Tanaka T, Kanegane H, Miyawaki T, Emi M, Kanakura Y, Kawase I, Naka T, Kishimoto T. |
| タイトル | Suppressor of cytokine signalling-1 gene silencing in acute myeloid leukaemia and human haematopoietic cell lines. |
| ジャーナル | Br J Haematol |
| Abstract |
The aim of this study was to investigate whether the suppressor of cytokine signalling (SOCS)-1 can act as a tumour suppressor when functioning as a negative regulator of the Janus family tyrosine kinases (JAKs), which have been reported to play important roles in leukaemogenesis. For this purpose, we carried out molecular analysis of the SOCS-1 gene in human acute myeloid leukaemia (AML) and human haematopoietic cell lines. Sequencing alterations in the coding region were found in two of 90 primary AML samples and one of 17 cell lines. Hypermethylation of the SOCS-1 gene was also observed in 72% of primary cases and 52% of cell lines and aberrant methylation strongly correlated with reduced expression. Transfection of SOCS-1 into Jurkat cells harbouring the mutation and methylation suppressed cell growth at a low serum concentration. These findings indicate that SOCS-1 is frequently silenced in haematopoietic malignancies, mainly as a result of hypermethylation, and suggest that SOCS-1 may be able to function as a tumour suppressor. |
| 巻・号 | 126(5) |
| ページ | 726-35 |
| 公開日 | 2004-9-1 |
| DOI | 10.1111/j.1365-2141.2004.05107.x |
| PII | BJH5107 |
| PMID | 15327527 |
| MeSH | Acute Disease Carrier Proteins / genetics* Cell Line Cell Line, Tumor Gene Silencing Genes, Tumor Suppressor* Hematologic Neoplasms / metabolism Hematopoiesis / physiology Humans Intracellular Signaling Peptides and Proteins* Jurkat Cells Leukemia, Myeloid / metabolism* Methylation Repressor Proteins / genetics* Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Suppressor of Cytokine Signaling 1 Protein Suppressor of Cytokine Signaling Proteins |
| IF | 5.518 |
| 引用数 | 61 |
| WOS 分野 | HEMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) |