RRC ID 19342
Author Cho SW, Yang JY, Sun HJ, Jung JY, Her SJ, Cho HY, Choi HJ, Kim SW, Kim SY, Shin CS.
Title Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells.
Journal Bone
Abstract Wnt inhibitory factor (WIF)-1 belongs to the members of secreted modulators of Wnt proteins. Secreted frizzled-related proteins (sFRPs), another member of Wnt modulators, have been shown to play differential roles in Wnt signaling depending on the subtypes and cell models. This study was undertaken to investigate the functional role of WIF-1 in osteoblastic differentiation of mouse mesenchymal C3H10T1/2 cells. C3H10T1/2 cells express endogenous WIF-1 and its expression level decreases during osteoblastogenesis. Treatment of C3H10T1/2 cells with WIF-1 significantly reduced alkaline phosphatase (ALP) activities induced by either osteogenic medium (OM, ascorbic acid and beta-glycerophosphate) or Wnt-3a conditioned medium (CM) in a dose-dependent manner. In contrast, the expression level of endogenous WIF-1 increased during adipogenesis and WIF-1 treatment resulted in increased adipogenesis. C3H10T1/2 cells transduced with WIF-1 retrovirus also exhibited reduced ALP activity and decreased mRNA expression of Runx2, collagen type 1, ALP and osteocalcin during osteoblastic differentiation compared to empty virus-transduced cells. Moreover, treatment with WIF-1 dose-dependently attenuates beta-catenin/T-cell factor (TCF) transcriptional activity in this cell line. Finally, knockdown of WIF-1 in C3H10T1/2 cells by RNA interference leads to increase in ALP activities. Collectively, these results indicate that WIF-1 plays as a negative regulator of osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells in vitro.
Volume 44(6)
Pages 1069-77
Published 2009-6-1
DOI 10.1016/j.bone.2009.02.012
PII S8756-3282(09)00452-9
PMID 19254785
MeSH 3T3-L1 Cells Adaptor Proteins, Signal Transducing Adipogenesis / drug effects Adipogenesis / genetics Alkaline Phosphatase / metabolism Animals Blotting, Western Cell Differentiation / drug effects* Cell Differentiation / genetics Cell Line Collagen Type I / genetics Core Binding Factor Alpha 1 Subunit / genetics Enzyme Activation / drug effects Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / pharmacology Extracellular Matrix Proteins / physiology* Humans Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / pharmacology Intercellular Signaling Peptides and Proteins / physiology* Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects* Mesenchymal Stem Cells / metabolism Mice Osteoblasts / cytology* Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / genetics Osteogenesis / drug effects Osteogenesis / genetics RNA Interference Reverse Transcriptase Polymerase Chain Reaction TCF Transcription Factors / genetics Transfection beta Catenin / genetics
IF 4.147
Times Cited 35
Human and Animal Cells MC3T3-E1(RCB1126)