論文 - 詳細
| RRC ID | 19345 |
|---|---|
| 著者 | Gómez R, Lago F, Gómez-Reino JJ, Dieguez C, Gualillo O. |
| タイトル | Expression and modulation of ghrelin O-acyltransferase in cultured chondrocytes. |
| ジャーナル | Arthritis Rheum |
| Abstract |
OBJECTIVE:To use reverse transcription-polymerase chain reaction to detect ghrelin O-acyltransferase (GOAT) transcripts in both murine and human chondrocytes, to evaluate the effect of pharmacologic in vitro treatments with lipopolysaccharide (LPS), growth hormone, ghrelin, and dexamethasone on GOAT messenger RNA (mRNA) expression, and to study the GOAT mRNA profile during chondrocyte differentiation. METHODS:Murine and human GOAT and ghrelin mRNA levels were determined by the SYBR Green-based quantitative real-time polymerase chain reaction method. RESULTS:GOAT mRNA was expressed in murine cartilage explants as well as in the cultured murine chondrogenic ATDC-5 cell line. GOAT was also expressed in human immortalized chondrocyte cell lines and in human cultured primary chondrocytes. In addition, GOAT mRNA expression in differentiating ATDC-5 cells was lower at the early stage of differentiation (days 3-7), whereas GOAT mRNA levels increased progressively at the late stages. Finally, among the drugs and hormones tested, only LPS was able to strongly decrease GOAT mRNA expression. CONCLUSION:These data indicate that chondrocytes are equipped with biochemical machinery for the synthesis of acylated ghrelin and suggest a novel role of the ghrelin axis in prehypertrophic and hypertrophic chondrocyte differentiation during endochondral ossification. |
| 巻・号 | 60(6) |
| ページ | 1704-9 |
| 公開日 | 2009-6-1 |
| DOI | 10.1002/art.24522 |
| PMID | 19479831 |
| MeSH | Acyltransferases / genetics Acyltransferases / metabolism* Animals Cell Differentiation / drug effects Cell Differentiation / genetics Cell Differentiation / physiology* Cell Line Chondrocytes / cytology* Chondrocytes / drug effects Chondrocytes / metabolism* Dexamethasone / pharmacology Gene Expression Regulation / drug effects Ghrelin / metabolism Ghrelin / pharmacology Growth Hormone / pharmacology Humans Lipopolysaccharides / pharmacology Membrane Proteins Mice Models, Animal RNA, Messenger / metabolism |
| 引用数 | 32 |
| WOS 分野 | RHEUMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |