| RRC ID |
19351
|
| 著者 |
Yang JY, Jung JY, Cho SW, Choi HJ, Kim SW, Kim SY, Kim HJ, Jang CH, Lee MG, Han J, Shin CS.
|
| タイトル |
Chloride intracellular channel 1 regulates osteoblast differentiation.
|
| ジャーナル |
Bone
|
| Abstract |
We have identified chloride intracellular channel 1 (CLIC1) through proteomic approach, which was increased in response to canonical wnt signaling while being almost shut-off by adipogenic treatment in mouse mesenchymal C3H10T1/2 cells. We found that CLIC1 was expressed in mouse (MC3T3-E1), rat (ROS 17/2.8 and UMR-106) or human (MG63 and SaOS2) osteoblastic cell lines as well as primary culture of mouse calvarial cells by RT-PCR or Western blot analysis. The expression level of CLIC1 is increased upon treatment of osteogenic medium, whereas it almost disappeared in adipogenic condition, confirming the proteomic data. The expression of CLIC1 was localized mainly in nuclear membrane and vesiculo-cytoplasmic, the latter of which was colocalized with mitochondria. Retroviral overexpression of CLIC1 did not increase whole-cell current but induces hyperpolarization of mitochondrial membrane potential estimated using the fluorescent dye TMRE. Moreover, overexpression of CLIC1 resulted in increase in osteoblastic differentiation of C3H10T1/2 cells as measured by ALP activities or osteoblastic gene expression (osterix, ALP and osteocalcin), although it did not result in induction of Runx2 transcription activities at mouse osteocalcin (OG2) promoter. Finally, in vitro knock-down of CLIC1 using stable siRNA CLIC1 significantly suppressed osteoblastic differentiation. Taken together, these results suggest that CLIC1 may play a role in the regulation of osteoblastic differentiation from mesenchymal progenitors, although its physiologic role in osteoblasts remains to be determined.
|
| 巻・号 |
45(6)
|
| ページ |
1175-85
|
| 公開日 |
2009-12-1
|
| DOI |
10.1016/j.bone.2009.08.012
|
| PII |
S8756-3282(09)01840-7
|
| PMID |
19703605
|
| MeSH |
Adipogenesis
Alkaline Phosphatase / antagonists & inhibitors
Alkaline Phosphatase / metabolism
Animals
Cell Differentiation*
Cell Line
Cell Membrane / metabolism
Chloride Channels / genetics
Chloride Channels / metabolism*
Culture Media
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation
Humans
Membrane Potential, Mitochondrial
Mice
Osteoblasts / cytology*
Osteoblasts / metabolism*
Osteogenesis
Protein Transport
RNA, Small Interfering / metabolism
Rats
Retroviridae / genetics
Signal Transduction
Subcellular Fractions / metabolism
Wnt Proteins / metabolism
|
| IF |
4.147
|
| 引用数 |
16
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
