| RRC ID |
19366
|
| 著者 |
Kobayashi M, Terawaki Y, Saito K, Kasuga K, Kojima I.
|
| タイトル |
Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro.
|
| ジャーナル |
Zygote
|
| Abstract |
The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain mouse strains during in vitro culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr <10000 fraction inhibited the development of 2-cell embryos to the 4- to 8-cell stages (57.7%), although the inhibition by this fraction was weaker than by the Reuber Mr <10000 fraction (19.8%). As a result of reversed-phase column chromatography, a 2-cell stage specific inhibitor of the cleavage of mouse embryos (Fr.B-25), which separated into the Mr <10000 fraction of the Reuber CM, was detected at a low level in the BRL Mr <10000 fraction. On the other hand, the Mr >10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the beta-A or beta-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.
|
| 巻・号 |
17(2)
|
| ページ |
169-74
|
| 公開日 |
2009-5-1
|
| DOI |
10.1017/S0967199408005121
|
| PII |
S0967199408005121
|
| PMID |
19187567
|
| MeSH |
Activins / metabolism*
Animals
Carcinoma, Hepatocellular
Cell Line, Tumor
Culture Media, Conditioned / pharmacology
Embryo, Mammalian / drug effects*
Embryo, Mammalian / physiology
Embryonic Development / drug effects
Female
Mice
Rats
Zygote / physiology*
|
| IF |
1.257
|
| 引用数 |
4
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
BRL(RCB0273) |
