| RRC ID |
19371
|
| Author |
Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Yamashita Y.
|
| Title |
Involvement of Ca(2+) in globular adiponectin-induced reactive oxygen species.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Globular adiponectin (gAd) induces the generation of reactive oxygen species (ROS) and nitric oxide (NO) in the murine macrophage cell line RAW 264. We investigated the role of Ca(2+) in gAd-induced ROS and NO generation. Pretreatment with BAPTA-AM, a selective chelator of intracellular Ca(2+) ([Ca(2+)](i)), partially reduced gAd-induced generation of ROS and NO in gAd-treated RAW 264 cells. The lowest [Ca(2+)](i) occurred 30min after gAd treatment, after which [Ca(2+)](i) increased continually and exceeded the initial level. The mitochondrial Ca(2+) ([Ca(2+)](m)) detected by Rhod-2 fluorescence started to increase at 6h after gAd treatment. Pretreatment with a NAD(P)H oxidase inhibitor, diphenyleneiodonium, prevented the reduction of [Ca(2+)](i) in the early phase after gAd treatment. Calcium depletion by BAPTA-AM had no effect on the gAd-induced [Ca(2+)](m) oscillation. The administration of a specific calmodulin inhibitor, calmidazolium, significantly suppressed gAd-induced ROS and NO generation and NOS activity.
|
| Volume |
381(4)
|
| Pages |
649-53
|
| Published |
2009-4-17
|
| DOI |
10.1016/j.bbrc.2009.02.115
|
| PII |
S0006-291X(09)00389-1
|
| PMID |
19249286
|
| MeSH |
Adiponectin / metabolism
Animals
Calcium / metabolism*
Calmodulin / antagonists & inhibitors
Calmodulin / metabolism
Cell Line
Enzyme Inhibitors / pharmacology
Imidazoles / pharmacology
Mice
Mitochondria / metabolism
Nitric Oxide / biosynthesis
Reactive Oxygen Species / metabolism*
|
| IF |
2.985
|
| Times Cited |
1
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
