論文 - 詳細
| RRC ID | 19375 |
|---|---|
| 著者 | Tanimura A, Morita T, Nezu A, Shitara A, Hashimoto N, Tojyo Y. |
| タイトル | Use of Fluorescence Resonance Energy Transfer-based Biosensors for the Quantitative Analysis of Inositol 1,4,5-Trisphosphate Dynamics in Calcium Oscillations. |
| ジャーナル | J Biol Chem |
| Abstract |
Inositol 1,4,5-trisphosphate (IP(3)) is an intracellular messenger that elicits a wide range of spatial and temporal Ca(2+) signals, and this signaling versatility is exploited to regulate diverse cellular responses. In this study, we have developed a series of IP(3) biosensors that exhibit strong pH stability and varying affinities for IP(3), as well as a method for the quantitative measurement of cytosolic concentrations of IP(3) ([IP(3)](i)) in single living cells. We applied this method to elucidate IP(3) dynamics during agonist-induced Ca(2+) oscillations, and we demonstrated cell type-dependent differences in IP(3) dynamics, a nonfluctuating rise in [IP(3)](i) and repetitive IP(3) spikes during Ca(2+) oscillations in COS-7 cells and HSY-EA1 cells, respectively. The size of the IP(3) spikes in HSY-EA1 cells varied from 10 to 100 nm, and the [IP(3)](i) spike peak was preceded by a Ca(2+) spike peak. These results suggest that repetitive IP(3) spikes in HSY-EA1 cells are passive reflections of Ca(2+) oscillations, and are unlikely to be essential for driving Ca(2+) oscillations. In addition, the interspike periods of Ca(2+) oscillations that occurred during the slow rise in [IP(3)](i) were not shortened by the rise in [IP(3)](i), indicating that IP(3)-dependent and -independent mechanisms may regulate the frequency of Ca(2+) oscillations. The novel method described herein as well as the quantitative information obtained by using this method should provide a valuable and sound basis for future studies on the spatial and temporal regulations of IP(3) and Ca(2+). |
| 巻・号 | 284(13) |
| ページ | 8910-7 |
| 公開日 | 2009-3-27 |
| DOI | 10.1074/jbc.M805865200 |
| PII | S0021-9258(20)32436-4 |
| PMID | 19158094 |
| PMC | PMC2659248 |
| MeSH | Animals Biological Clocks* Biosensing Techniques / methods* COS Cells Calcium / analysis Calcium / metabolism* Calcium Signaling* Chlorocebus aethiops Fluorescence Resonance Energy Transfer / methods* Humans Inositol 1,4,5-Trisphosphate / analysis Inositol 1,4,5-Trisphosphate / metabolism* |
| IF | 4.238 |
| 引用数 | 51 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |