論文 - 詳細
| RRC ID | 1946 |
|---|---|
| 著者 | Takayanagi S, Hiroyama T, Yamazaki S, Nakajima T, Morita Y, Usui J, Eto K, Motohashi T, Shiomi K, Keino-Masu K, Masu M, Oike Y, Mori S, Yoshida N, Iwama A, Nakauchi H. |
| タイトル | Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain. |
| ジャーナル | Blood |
| Abstract |
Using an in silico database search, we identified a novel gene encoding a cell surface molecule with a thrombospondin domain, and designated the gene as transmembrane molecule with thrombospondin module (Tmtsp). Expression profiling of Tmtsp using specific monoclonal antibodies and Venus, a variant of yellow fluorescent protein knock-in mice in the Tmtsp locus, demonstrated its specific expression in hematopoietic and endothelial cells. In lymphohematopoietic cells, Tmtsp was predominantly expressed in hematopoietic stem and progenitor cells, and the level of expression gradually declined as the cells differentiated. Venus expression faithfully traced the expression of Tmtsp, and the level of Venus expression correlated well to the in vitro hematopoietic activity as well as the in vivo bone marrow repopulating capacity. Notably, Venus expression marked the development of definitive hematopoiesis in both the extraembryonic yolk sac and the intraembryonic aorta-gonad-mesonephros (AGM) region and, in combination with CD41, strikingly promoted the enrichment of developing progenitors in the CD41(+)Venus(high) fraction at embryonic day 10.5 (E10.5). In this context, Tmtsp is a novel marker gene for primitive hematopoietic cells and endothelial cells, and Tmtsp(Venus/)(+) mice would serve as a valuable mouse model for the analysis of both embryonic and adult hematopoiesis, as well as for vascular biology. |
| 巻・号 | 107(11) |
| ページ | 4317-25 |
| 公開日 | 2006-6-1 |
| DOI | 10.1182/blood-2005-09-3747 |
| PII | S0006-4971(20)64566-9 |
| PMID | 16455951 |
| MeSH | Amino Acid Sequence Animals Bone Marrow / physiology Embryo, Mammalian / cytology Endothelial Cells / chemistry* Gene Library Genetic Markers Hematopoiesis Hematopoietic Stem Cells / chemistry* Humans Membrane Proteins Mice Molecular Sequence Data Spleen Thrombospondin 1 / analysis Thrombospondin 1 / chemistry Thrombospondin 1 / genetics Thrombospondins / analysis Thrombospondins / chemistry Thrombospondins / genetics* Tissue Distribution |
| IF | 17.794 |
| 引用数 | 11 |
| WOS 分野 | HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |