| RRC ID |
1946
|
| 著者 |
Takayanagi S, Hiroyama T, Yamazaki S, Nakajima T, Morita Y, Usui J, Eto K, Motohashi T, Shiomi K, Keino-Masu K, Masu M, Oike Y, Mori S, Yoshida N, Iwama A, Nakauchi H.
|
| タイトル |
Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain.
|
| ジャーナル |
Blood
|
| Abstract |
Using an in silico database search, we identified a novel gene encoding a cell surface molecule with a thrombospondin domain, and designated the gene as transmembrane molecule with thrombospondin module (Tmtsp). Expression profiling of Tmtsp using specific monoclonal antibodies and Venus, a variant of yellow fluorescent protein knock-in mice in the Tmtsp locus, demonstrated its specific expression in hematopoietic and endothelial cells. In lymphohematopoietic cells, Tmtsp was predominantly expressed in hematopoietic stem and progenitor cells, and the level of expression gradually declined as the cells differentiated. Venus expression faithfully traced the expression of Tmtsp, and the level of Venus expression correlated well to the in vitro hematopoietic activity as well as the in vivo bone marrow repopulating capacity. Notably, Venus expression marked the development of definitive hematopoiesis in both the extraembryonic yolk sac and the intraembryonic aorta-gonad-mesonephros (AGM) region and, in combination with CD41, strikingly promoted the enrichment of developing progenitors in the CD41(+)Venus(high) fraction at embryonic day 10.5 (E10.5). In this context, Tmtsp is a novel marker gene for primitive hematopoietic cells and endothelial cells, and Tmtsp(Venus/)(+) mice would serve as a valuable mouse model for the analysis of both embryonic and adult hematopoiesis, as well as for vascular biology.
|
| 巻・号 |
107(11)
|
| ページ |
4317-25
|
| 公開日 |
2006-6-1
|
| DOI |
10.1182/blood-2005-09-3747
|
| PII |
S0006-4971(20)64566-9
|
| PMID |
16455951
|
| MeSH |
Amino Acid Sequence
Animals
Bone Marrow / physiology
Embryo, Mammalian / cytology
Endothelial Cells / chemistry*
Gene Library
Genetic Markers
Hematopoiesis
Hematopoietic Stem Cells / chemistry*
Humans
Membrane Proteins
Mice
Molecular Sequence Data
Spleen
Thrombospondin 1 / analysis
Thrombospondin 1 / chemistry
Thrombospondin 1 / genetics
Thrombospondins / analysis
Thrombospondins / chemistry
Thrombospondins / genetics*
Tissue Distribution
|
| IF |
17.794
|
| 引用数 |
11
|
|
WOS 分野
|
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
OP9(RCB1124) |
