論文 - 詳細
| RRC ID | 1960 |
|---|---|
| 著者 | Xie Q, Matsunaga S, Niimi S, Ogawa S, Tokuyasu K, Sakakibara Y, Machida S. |
| タイトル | Human lectin-like oxidized low-density lipoprotein receptor-1 functions as a dimer in living cells. |
| ジャーナル | DNA Cell Biol |
| Abstract |
Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a unique scavenger receptor that plays important roles in atherogenesis and has been thought to function as a monomer. Using coimmunoprecipitation studies, we demonstrate that human LOX-1 (hLOX-1) forms constitutive homo-interactions in vivo. Western blot analysis of cell lysates under nonreducing or reducing conditions revealed one clear immunoreactive species corresponding to the size of a putative receptor dimer or a monomer, respectively, consistent with the presence of disulfide-linked hLOX-1 complexes. Site-directed mutagenesis studies indicated that cysteine 140 has a key role in the formation of these disulfide-linked hLOX-1 dimers. Eliminating this intermolecular disulfide bond markedly impairs the recognition of Escherichia coli by hLOX-1. Furthermore, these dimers can act as a "structural unit" to form noncovalently associated oligomers, as demonstrated by a membrane-impermeant crosslinker, which resulted in immunoreactive species corresponding to the sizes of putative tetramers and hexamers. These results provide the first evidence for the existence of hLOX-1 dimers/oligomers. |
| 巻・号 | 23(2) |
| ページ | 111-7 |
| 公開日 | 2004-2-1 |
| DOI | 10.1089/104454904322759920 |
| PMID | 15000751 |
| MeSH | Animals Blotting, Western COS Cells Chlorocebus aethiops DNA, Complementary / genetics Dimerization Fluorescent Antibody Technique Humans Mutagenesis, Site-Directed Plasmids / genetics Precipitin Tests Receptors, LDL / genetics* Receptors, LDL / metabolism* |
| IF | 3.314 |
| 引用数 | 39 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |