RRC ID |
19607
|
著者 |
Noman AS, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
|
タイトル |
Thalidomide inhibits lipopolysaccharide-induced nitric oxide production and prevents lipopolysaccharide-mediated lethality in mice.
|
ジャーナル |
FEMS Immunol Med Microbiol
|
Abstract |
The effect of thalidomide on lipopolysaccharide-induced nitric oxide (NO) production was studied using RAW 264.7 macrophage-like cells. Thalidomide significantly inhibited lipopolysaccharide-induced NO production via reduced expression of an inducible NO synthase. Thalidomide reduced the phosphorylation of the p65 nuclear factor-kappaB subunit, inhibitory kappaB (IkappaB) and IkappaB kinase in lipopolysaccharide-stimulated cells. However, thalidomide did not affect the expression of interferon-beta (IFN-beta) and interferon regulatory factor-1 in response to lipopolysaccharide. Further, thalidomide inhibited the MyD88 augmentation in lipopolysaccharide-stimulated cells, whereas it did not alter the expression of TIR domain-containing adaptor-inducing IFN-beta in the MyD88-independent pathway. Thalidomide significantly inhibited the NO production in response to Pam(3)Cys, CpG DNA and imiquimod as MyD88-dependent Toll-like receptor (TLR) ligands, but not polyI:C as a MyD88-independent TLR ligand. Therefore, thalidomide was suggested to inhibit lipopolysaccharide-induced NO production via downregulation of the MyD88-dependent signal pathway. The anti-inflammatory action of thalidomide might be involved in the prevention of lipopolysaccharide-mediated lethality in mice.
|
巻・号 |
56(3)
|
ページ |
204-11
|
公開日 |
2009-8-1
|
DOI |
10.1111/j.1574-695X.2009.00567.x
|
PII |
FIM567
|
PMID |
19538513
|
MeSH |
Animals
Cell Line
Cell Survival
Down-Regulation
I-kappa B Kinase / metabolism
I-kappa B Proteins / metabolism
Immunosuppressive Agents / pharmacology*
Lipopolysaccharides / immunology*
Macrophages / drug effects
Macrophages / immunology
Mice
Mice, Inbred C57BL
Myeloid Differentiation Factor 88 / antagonists & inhibitors
Nitric Oxide / antagonists & inhibitors*
Nitric Oxide Synthase / antagonists & inhibitors
Phosphorylation
Survival Analysis
Thalidomide / pharmacology*
Transcription Factor RelA / metabolism
|
引用数 |
10
|
WOS 分野
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |