論文 - 詳細
| RRC ID | 19628 |
|---|---|
| 著者 | Luo JC, Cho CH, Ng KM, Hsiang KW, Lu CL, Chen TS, Chang FY, Lin HC, Perng CL, Lee SD. |
| タイトル | Dexamethasone inhibits tumor necrosis factor-alpha-stimulated gastric epithelial cell migration. |
| ジャーナル | J Chin Med Assoc |
| Abstract |
BACKGROUND:Cell migration (restitution) occurs in the early phase of gastric ulcer healing. Tumor necrosis factor (TNF)-alpha is overexpressed at the ulcer margin and plays a physiologic role in gastric ulcer healing. Dexamethasone, which is a potent corticosteroid, delays rat gastric ulcer healing. We evaluated whether dexamethasone inhibited TNF-alpha-stimulated gastric epithelial cell migration using a rat normal gastric epithelial cell line (RGM-1). METHODS:An artificial wound model was employed to measure cell migration. Western blot was performed to evaluate the possible mechanisms. Intracellular prostaglandin E2 level was measured using an enzyme-linked immunosorbent assay. RESULTS:TNF-alpha treatment (10 ng/mL) for 12-48 hours significantly increased RGM-1 cell migration, and TNF-alpha treatment increased cyclooxygenase (COX)-2 protein expression 8 hours later and prostaglandin E2 (PGE2) synthesis 12 hours later compared with control (p < 0.05). Dexamethasone (10(-6) M) significantly inhibited the stimulatory effect of TNF-alpha on RGM-1 cell migration, which was associated with a significant decrease in COX-2 expression and PGE2 level in cells (p < 0.05). CONCLUSION:TNF-alpha plays a regulatory role in rat gastric epithelial cell migration and dexamethasone inhibited TNF-alpha-stimulated cell migration, which was associated with a decrease in COX-2 expression and PGE2 formation. |
| 巻・号 | 72(10) |
| ページ | 509-14 |
| 公開日 | 2009-10-1 |
| DOI | 10.1016/S1726-4901(09)70419-8 |
| PII | S1726-4901(09)70419-8 |
| PMID | 19837644 |
| MeSH | Animals Cell Movement / drug effects Cells, Cultured Dexamethasone / pharmacology* Dinoprostone / analysis Fibroblast Growth Factor 2 / analysis Gastric Mucosa / chemistry Gastric Mucosa / cytology Gastric Mucosa / drug effects* Prostaglandin-Endoperoxide Synthases / analysis Rats Rats, Wistar Tumor Necrosis Factor-alpha / antagonists & inhibitors* |
| IF | 2.17 |
| 引用数 | 9 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |