| RRC ID |
19633
|
| 著者 |
Hashimoto Y, Adachi S, Matsuno T, Omata K, Yoshitaka Y, Ozeki Y, Umezu Y, Satoh T, Nakamura M.
|
| タイトル |
Effect of an injectable 3D scaffold for osteoblast differentiation depends on bead size.
|
| ジャーナル |
Biomed Mater Eng
|
| Abstract |
The objective of this study was to evaluate the effect of beta-tricalcium phosphate (beta-TCP) bead size on the behavior of KUSA/A1 mouse osteoblasts when the beta-TCP beads are used as the solid phase of a scaffold in which alginate was used as the gel phase. KUSA/A1 cells were loaded onto a three-dimensional (3D) scaffold fabricated from beta-TCP beads with diameters ranging from 300 to 500 microm (small beads), 500-700 microm (medium beads) and 700-850 microm (large beads); cells were cultured for 3, 7 and 14 days. Scanning electron microscope observations showed that each bead was connected in a network consisting of the alginate gel and KUSA/A1 cellular matrix that was tightly bonded to form a 3D structure. After 3 days, cells in the 3D scaffold with medium beads had a significantly higher alkaline phosphatase activity (ALP) than cells in the other scaffolds. However, by 7 and 14 days in culture there was no significant difference in DNA levels, ALP activity or osteocalcin expression. At 8 weeks, only the composite containing small beads and KUSA/A1 cells had turned completely into bone in vivo. Thus, bead size may influence the success of bone formation in this context.
|
| 巻・号 |
19(6)
|
| ページ |
391-400
|
| 公開日 |
2009-1-1
|
| DOI |
10.3233/BME-2009-0604
|
| PII |
67L7G57114324514
|
| PMID |
20231791
|
| MeSH |
Animals
Bone Substitutes / administration & dosage
Bone Substitutes / chemistry*
Calcium Phosphates / administration & dosage
Calcium Phosphates / chemistry*
Cell Differentiation
Cell Line
Cells, Cultured
Guided Tissue Regeneration / instrumentation*
Guided Tissue Regeneration / methods
Injections
Materials Testing
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / physiology
Mice
Mice, Nude
Osteoblasts / cytology*
Osteoblasts / physiology
Osteogenesis / physiology*
Tissue Scaffolds*
|
| IF |
1.243
|
| 引用数 |
5
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
KUSA-A1(RCB2081) |
