RRC ID 19670
Author Imai KS, Daido Y, Kusakabe TG, Satou Y.
Title Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos.
Journal Science
Abstract The function of bone morphogenetic protein (BMP) signaling in dorsoventral (DV) patterning of animal embryos is conserved among Bilateria. In vertebrates, the BMP ligand antidorsalizing morphogenetic protein (Admp) is expressed dorsally and moves to the opposite side to specify the ventral fate. Here, we show that Pinhead is an antagonist specific for Admp with a role in establishing the DV axis of the trunk epidermis in embryos of the ascidian Ciona intestinalis. Pinhead and Admp exist in tandem in the genomes of various animals from arthropods to vertebrates. This genomic configuration is important for mutually exclusive expression of these genes, because Pinhead transcription directly disturbs the action of the Admp enhancer. Our data suggest that this dual negative regulatory mechanism is widely conserved in animals.
Volume 337(6097)
Pages 964-7
Published 2012-8-24
DOI 10.1126/science.1222488
PII 337/6097/964
PMID 22923581
MeSH Amino Acid Sequence Animals Body Patterning* Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism Bone Morphogenetic Protein 4 / genetics Bone Morphogenetic Protein 4 / metabolism Bone Morphogenetic Proteins / chemistry Bone Morphogenetic Proteins / genetics* Bone Morphogenetic Proteins / metabolism Ciona intestinalis / embryology* Ciona intestinalis / genetics Ciona intestinalis / metabolism Embryo, Nonmammalian / metabolism* Embryonic Development Enhancer Elements, Genetic Epidermis / embryology Gastrula / metabolism Gene Expression Regulation, Developmental* Molecular Sequence Data Oligodeoxyribonucleotides, Antisense Oryzias / embryology Oryzias / genetics Oryzias / metabolism Promoter Regions, Genetic Signal Transduction Transcription, Genetic*
IF 41.063
Times Cited 9
C.intestinalis / (O.japonicus) Wild C. int