論文 - 詳細
| RRC ID | 19690 |
|---|---|
| 著者 | Xu Y, Quinn CC. |
| タイトル | MIG-10 functions with ABI-1 to mediate the UNC-6 and SLT-1 axon guidance signaling pathways. |
| ジャーナル | PLoS Genet |
| Abstract |
Extracellular guidance cues steer axons towards their targets by eliciting morphological changes in the growth cone. A key part of this process is the asymmetric recruitment of the cytoplasmic scaffolding protein MIG-10 (lamellipodin). MIG-10 is thought to asymmetrically promote outgrowth by inducing actin polymerization. However, the mechanism that links MIG-10 to actin polymerization is not known. We have identified the actin regulatory protein ABI-1 as a partner for MIG-10 that can mediate its outgrowth-promoting activity. The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. Dosage sensitive genetic interactions indicate that MIG-10 functions with ABI-1 and WVE-1 to mediate axon guidance. Epistasis analysis reveals that ABI-1 and WVE-1 function downstream of MIG-10 to mediate its outgrowth-promoting activity. Moreover, experiments with cultured mammalian cells suggest that the interaction between MIG-10 and ABI-1 mediates a conserved mechanism that promotes formation of lamellipodia. Together, these observations suggest that MIG-10 interacts with ABI-1 and WVE-1 to mediate the UNC-6 and SLT-1 guidance pathways. |
| 巻・号 | 8(11) |
| ページ | e1003054 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pgen.1003054 |
| PII | PGENETICS-D-12-00453 |
| PMID | 23209429 |
| PMC | PMC3510047 |
| MeSH | Animals Axons / metabolism Axons / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Movement Cytoskeletal Proteins* / genetics Cytoskeletal Proteins* / metabolism Gene Expression Regulation, Developmental Humans Nerve Tissue Proteins* / genetics Nerve Tissue Proteins* / metabolism Netrins Neurogenesis* Signal Transduction |
| IF | 5.175 |
| 引用数 | 5 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | abi-1(tm494) |