RRC ID 19690
著者 Xu Y, Quinn CC.
タイトル MIG-10 functions with ABI-1 to mediate the UNC-6 and SLT-1 axon guidance signaling pathways.
ジャーナル PLoS Genet
Abstract Extracellular guidance cues steer axons towards their targets by eliciting morphological changes in the growth cone. A key part of this process is the asymmetric recruitment of the cytoplasmic scaffolding protein MIG-10 (lamellipodin). MIG-10 is thought to asymmetrically promote outgrowth by inducing actin polymerization. However, the mechanism that links MIG-10 to actin polymerization is not known. We have identified the actin regulatory protein ABI-1 as a partner for MIG-10 that can mediate its outgrowth-promoting activity. The SH3 domain of ABI-1 binds to MIG-10, and loss of function of either of these proteins causes similar axon guidance defects. Like MIG-10, ABI-1 functions in both the attractive UNC-6 (netrin) pathway and the repulsive SLT-1 (slit) pathway. Dosage sensitive genetic interactions indicate that MIG-10 functions with ABI-1 and WVE-1 to mediate axon guidance. Epistasis analysis reveals that ABI-1 and WVE-1 function downstream of MIG-10 to mediate its outgrowth-promoting activity. Moreover, experiments with cultured mammalian cells suggest that the interaction between MIG-10 and ABI-1 mediates a conserved mechanism that promotes formation of lamellipodia. Together, these observations suggest that MIG-10 interacts with ABI-1 and WVE-1 to mediate the UNC-6 and SLT-1 guidance pathways.
巻・号 8(11)
ページ e1003054
公開日 2012-1-1
DOI 10.1371/journal.pgen.1003054
PII PGENETICS-D-12-00453
PMID 23209429
PMC PMC3510047
MeSH Animals Axons / metabolism Axons / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Movement Cytoskeletal Proteins* / genetics Cytoskeletal Proteins* / metabolism Gene Expression Regulation, Developmental Humans Nerve Tissue Proteins* / genetics Nerve Tissue Proteins* / metabolism Netrins Neurogenesis* Signal Transduction
IF 5.175
引用数 5
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 abi-1(tm494)