| RRC ID |
19696
|
| 著者 |
Xu GH, Su WY, Shu YJ, Cong WW, Wu L, Guo CH.
|
| タイトル |
RAPD and ISSR-assisted identification and development of three new SCAR markers specific for the Thinopyrum elongatum E (Poaceae) genome.
|
| ジャーナル |
Genet Mol Res
|
| Abstract |
Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We used 36 RAPD primers and 33 ISSR primers to screen for polymorphisms in the common wheat variety Chinese Spring and in T. elongatum. Two RAPD markers and one ISSR marker, designated OPF03(1407), LW10(1487) and UBC841(701), were identified and were specific for the T. elongatum E genome. Three pairs of primers flanking these specific sequences were designed to produce SCAR markers. All three SCAR markers were T. elongatum E genome-specific. Two of these SCAR markers, SCAR(807) and SCAR(577), were present in all seven T. elongatum chromosomes, while SCAR(839) was specific for T. elongatum chromosomes 2E and 3E. These newly developed SCAR markers should be useful for detecting alien genome chromatin or chromosome segments in the genetic background of common wheat.
|
| 巻・号 |
11(2)
|
| ページ |
1741-51
|
| 公開日 |
2012-6-29
|
| DOI |
10.4238/2012.June.29.7
|
| PII |
gmr1595
|
| PMID |
22843051
|
| MeSH |
Base Sequence
Consensus Sequence
Genes, Plant*
Genetic Markers
Genome, Plant
Microsatellite Repeats*
Molecular Sequence Data
Poaceae / genetics*
Random Amplified Polymorphic DNA Technique
Reproducibility of Results
Sequence Analysis, DNA
|
| 引用数 |
13
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| コムギ |
TACBOW0190 (CS-2C disomic addition line)
TACBOW0120 Thinopyrum elongatum (2x)
TACBOW0038 (21’’+1E’’)
TACBOW0039 (21’’+2E’’)
TACBOW0040 (21’’+3E’’)
TACBOW0041 (21’’+4E’’)
TACBOW0043 (21’’+5E’’)
TACBOW0042 (21’’+6E’’)
TACBOW0044 (21’’+7E’’) |
