| RRC ID |
19699
|
| 著者 |
Schubert V, Meister A, Tsujimoto H, Endo TR, Houben A.
|
| タイトル |
Similar rye A and B chromosome organization in meristematic and differentiated interphase nuclei.
|
| ジャーナル |
Chromosome Res
|
| Abstract |
Supernumerary (B) chromosomes of rye are not required for plant development and exhibit a reduced transcription activity. These special features inspired us to analyse whether there are differences between A and B chromatin organization in interphase nuclei. Applying fluorescence in situ hybridization, we found that both rye A and B chromosomes added to hexaploid wheat showed in meristematic nuclei a string-like shape and a clear Rabl orientation. In 4C differentiated leaf nuclei, a more relaxed chromatin structure, round-shaped chromosome territories and a less pronounced Rabl configuration were found. Also, the observed random association of homologues in 2C and 4C nuclei indicated in general a similar behaviour of A and B chromosomes. Whereas in differentiated 4C nuclei A sister centromeres are separated, B sister centromeres align in nearly all nuclei. In short, despite the different transcription activity of A and B chromosomes, both types of chromosomes exhibit a similar organization in meristematic and differentiated interphase nuclei. But the deletion of a B chromosome segment responsible for non-disjunction during gametogenesis induces released sister centromeres also in some interphase nuclei of somatic tissue. Hence, the control of rye B chromosome non-disjunction is also active in sporophytic cells.
|
| 巻・号 |
19(5)
|
| ページ |
645-55
|
| 公開日 |
2011-7-1
|
| DOI |
10.1007/s10577-011-9224-5
|
| PMID |
21674259
|
| MeSH |
Cell Differentiation
Cell Nucleus / genetics*
Chromatids / genetics
Chromosome Segregation
Chromosomes, Plant / genetics*
In Situ Hybridization, Fluorescence
Interphase / genetics*
Meristem / cytology
Meristem / genetics*
Microscopy, Fluorescence
Nondisjunction, Genetic
Plant Roots / cytology
Plant Roots / genetics
Secale / genetics
Triticum / genetics
|
| IF |
3.413
|
| 引用数 |
7
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| コムギ |
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