Reference - Detail
| RRC ID | 19703 |
|---|---|
| Author | Danilova TV, Friebe B, Gill BS. |
| Title | Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. |
| Journal | Chromosoma |
| Abstract |
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation. |
| Volume | 121(6) |
| Pages | 597-611 |
| Published | 2012-12-1 |
| DOI | 10.1007/s00412-012-0384-7 |
| PMID | 23052335 |
| MeSH | Acetyl-CoA Carboxylase / genetics* Chromosome Mapping* Chromosomes, Plant DNA, Complementary Diploidy Genome, Plant* In Situ Hybridization, Fluorescence / methods* Plant Proteins / genetics Tetraploidy Triticum / genetics* |
| IF | 3.442 |
| Times Cited | 53 |
| WOS Category | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Wheat | tplb0013a02 tplb0029f23 tplb0048d21 tplb0011e24 tplb0004j16 tplb0017g02 tplb0033b21 tplb0043p15 tplb0027f03 |