論文 - 詳細
| RRC ID | 19706 |
|---|---|
| 著者 | Saito T, Shiba K, Inaba K, Yamada L, Sawada H. |
| タイトル | Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Many hermaphroditic organisms possess a self-incompatibility system to avoid self-fertilization. Recently, we identified the genes responsible for self-sterility in a hermaphroditic primitive chordate (ascidian), Ciona intestinalis: sperm-side polycystin 1-like receptors s-Themis-A/B and egg-side fibrinogen-like ligands on the vitelline coat (VC) v-Themis-A/B. Here, we investigated the sperm behavior and intracellular Ca(2+) concentration ([Ca(2+)](i)) in response to self/nonself-recognition. We found that sperm motility markedly decreased within 5 min after attachment to the VC of self-eggs but not after attachment to the VC of nonself-eggs and that the apparent decrease in sperm motility was suppressed in low Ca(2+) seawater. High-speed video analysis revealed that sperm detached from the self-VC or stopped motility within 5 min after binding to the self-VC. Because s-Themis-B contains a cation channel domain in its C terminus, we monitored sperm [Ca(2+)](i) by real-time [Ca(2+)](i) imaging using Fluo-8H-AM (AAT Bioquest, Inc.). Interestingly, we found that sperm [Ca(2+)](i) rapidly and dramatically increased and was maintained at a high level in the head and flagellar regions when sperm interacted with the self-VC but not when the sperm interacted with the nonself-VC. The increase in [Ca(2+)](i) was also suppressed by low-Ca(2+) seawater. These results indicate that the sperm self-recognition signal triggers [Ca(2+)](i) increase and/or Ca(2+) influx, which elicits a self-incompatibility response to reject self-fertilization in C. intestinalis. |
| 巻・号 | 109(11) |
| ページ | 4158-62 |
| 公開日 | 2012-3-13 |
| DOI | 10.1073/pnas.1115086109 |
| PII | 1115086109 |
| PMID | 22357759 |
| PMC | PMC3306710 |
| MeSH | Animals Calcium / metabolism* Calcium Signaling Ciona intestinalis / physiology* Male Models, Biological Ovum / cytology Ovum / metabolism Rotation Self-Fertilization / physiology* Sperm Motility Spermatozoa / metabolism* Vitelline Membrane / metabolism |
| IF | 9.412 |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |