RRC ID 1983
Author Wu WK, Li GR, Wong HP, Hui MK, Tai EK, Lam EK, Shin VY, Ye YN, Li P, Yang YH, Luo JC, Cho CH.
Title Involvement of Kv1.1 and Nav1.5 in proliferation of gastric epithelial cells.
Journal J. Cell. Physiol.
Abstract In the present study, patch clamp experiments demonstrated the expression of multiple ionic currents, including a Ba2+-sensitive inward rectifier K+ current (IKir), a 4-aminopyridine- (4-AP) sensitive delayed rectifier K+ current (IKDR), and a nifedipine-sensitive, tetrodotoxin-resistant inward Na+ current (INa.TTXR) in the non-transformed rat gastric epithelial cell line RGM-1. RT-PCR revealed molecular identities of mRNAs for the functional ionic currents, including Kir1.2 for IKir, Kv1.1, Kv1.6, and Kv2.1 for IKDR, and Nav1.5 for INa.TTXR. Pharmacologic blockade of Kv and Nav, but not Kir, suppressed RGM-1 cell proliferation. To further elucidate which subtypes of the ion channels were involved in cell proliferation, RNA interference was employed to knockdown specific gene expression. Downregulation of Kv1.1 or Nav1.5 by RNA interference suppressed RGM-1 cell proliferation. To conclude, our study is the first to delineate the expression of ion channels and their functions as growth modulators in gastric epithelial cells.
Volume 207(2)
Pages 437-44
Published 2006-5
DOI 10.1002/jcp.20576
PMID 16331678
MeSH 4-Aminopyridine / pharmacology Animals Barium / pharmacology Cell Line Cell Proliferation / drug effects* Epithelial Cells / cytology Epithelial Cells / drug effects Epithelial Cells / physiology* Gastric Mucosa / cytology Gene Expression / genetics Kv1.1 Potassium Channel / genetics Kv1.1 Potassium Channel / physiology* NAV1.5 Voltage-Gated Sodium Channel Nifedipine / pharmacology Patch-Clamp Techniques Potassium / metabolism Potassium Channels / genetics Potassium Channels / physiology RNA, Small Interfering / genetics Rats Sodium / metabolism Sodium Channels / genetics Sodium Channels / physiology*
IF 4.522
Times Cited 18
Human and Animal Cells RGM1