| RRC ID |
2000
|
| 著者 |
Yanagisawa A, Endo C, Okawa K, Shitara S, Kugoh H, Kakitani M, Oshimura M, Tomizuka K.
|
| タイトル |
Generation of chromosome-specific monoclonal antibodies using in vitro-differentiated transchromosomic mouse embryonic stem cells.
|
| ジャーナル |
Stem Cells
|
| Abstract |
Monoclonal antibodies (MoAbs) recognizing lineage- and stage-specific human cell-surface antigens are valuable reagents for the characterization and isolation of various specialized cell populations derived from human embryonic stem cells (hESCs). In this report, we examined the use of in vitro differentiated transchromosomic mouse embryonic stem cells (TC-ESCs) as immunogens to obtain MoAbs against human cell-surface antigens. Immunization of a neural-cell population derived from differentiating human chromosome 4 and 11 TC-ESCs resulted in two chromosome-specific MoAbs, h4-neural1 and h11-neural1, respectively. The staining profiles of differentiated TC-ESCs and human embryonic carcinoma cells with these MoAbs were similar to the expression profile of nestin, a well-characterized intracellular marker for neural progenitor cells. We also described the successful purification and identification of the gene for h4-neural1 antigen (CD133, 4p15.32) with immunoaffinity chromatography. This procedure may have significant utility in generating MoAbs useful for understanding the mechanism that regulates the in vitro differentiation of hESCs.
|
| 巻・号 |
23(10)
|
| ページ |
1479-88
|
| 公開日 |
2005-1-1
|
| DOI |
10.1634/stemcells.2004-0369
|
| PII |
2004-0369
|
| PMID |
16081665
|
| MeSH |
AC133 Antigen
Animals
Antibodies, Monoclonal / biosynthesis*
Antigens, CD / genetics
Antigens, CD / metabolism*
Antigens, Surface / genetics
Antigens, Surface / metabolism
CD56 Antigen / genetics
CD56 Antigen / metabolism*
Cell Differentiation*
Cells, Cultured
Chromosomes, Mammalian / genetics
Chromosomes, Mammalian / immunology*
Embryo Research
Glycoproteins / genetics
Glycoproteins / metabolism*
Humans
Mice
Neurons / cytology
Peptides / genetics
Peptides / metabolism*
Stem Cells / cytology*
Transgenes*
|
| IF |
6.022
|
| 引用数 |
2
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
HEMATOLOGY
ONCOLOGY
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| ヒト・動物細胞 |
NEC8(RCB0489)
NEC14(RCB0490)
HMV-II(RCB0777) |
