論文 - 詳細
| RRC ID | 20053 |
|---|---|
| 著者 | Okamoto T, Kim D, Oda T, Matsuoka K, Ishimatsu A, Muramatsu T. |
| タイトル | Concanavalin A-induced discharge of glycocalyx of raphidophycean flagellates, Chattonella marina and Heterosigma akashiwo. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Chattonella marina and Heterosigma akashiwo, known as red tide phytoplankton, are naturally wall-less and have quite fragile cell structures. In this study, we found that an equilibrium dialysis technique allowed the study of lectin binding to these flagellates. The results suggested that concanavalin A (Con A) binds to these flagellate cells through the specific carbohydrate moieties on the cell surface. Interestingly, the binding of an excess of Con A on the cell surface caused morphological changes concomitant with discharge of glycocalyx, a polysaccharide-containing common structure on the external cell surface of these flagellates. Fluorescent microscopic observation using FITC-labeled Con A (F-Con A) confirmed that F-Con A molecules are localized on the discharged glycocalyx. |
| 巻・号 | 64(8) |
| ページ | 1767-70 |
| 公開日 | 2000-8-1 |
| DOI | 10.1271/bbb.64.1767 |
| PMID | 10993174 |
| MeSH | Animals Concanavalin A / pharmacology* Dinoflagellida / drug effects* Dinoflagellida / metabolism Fluorescein-5-isothiocyanate Glycocalyx / metabolism* Microscopy, Fluorescence Phytoplankton / drug effects* Phytoplankton / metabolism Surface Properties |
| IF | 1.516 |
| 引用数 | 8 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 藻類 | NIES-6 |