| RRC ID |
2007
|
| 著者 |
Fujiki Y, Ito M, Itoh T, Nishida I, Watanabe A.
|
| タイトル |
Activation of the promoters of Arabidopsis genes for the branched-chain alpha-keto acid dehydrogenase complex in transgenic tobacco BY-2 cells under sugar starvation.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
Sugar starvation exerted by sub-10 mM levels of sucrose on Arabidopsis T87 suspension-cultured cells triggered marked accumulation of the transcripts of genes for E1beta and E2 subunit of the branched-chain alpha-keto acid dehydrogenase complex. Similar levels of sugar starvation increased the luciferase activity in transgenic tobacco BY-2 lines expressing the Arabidopsis E1beta- or E2-promoter-luciferase fusion gene. These results indicate that sugar levels tightly regulate the E1beta and E2 promoter activity in the heterologous plant system. We further showed in the transgenic tobacco BY-2 lines that sugar-starvation-induced activation of the E1beta and E2 promoters was prevented by K-252a, an inhibitor of Ser/Thr protein kinase, and was enhanced by okadaic acid, an inhibitor of protein phosphatases. By contrast, the cauliflower mosaic virus 35S promoter activity in sugar-starved BY-2 cells was not significantly affected by K-252a and only slightly enhanced by okadaic acid. Taken together, we propose that transcriptional activation of genes for the branched-chain alpha-keto acid dehydrogenase complex and its modulation by specific protein kinases/phosphatases are of critical importance in branched-chain amino acid catabolism in plant cells under sugar starvation.
|
| 巻・号 |
43(3)
|
| ページ |
275-80
|
| 公開日 |
2002-3-1
|
| DOI |
10.1093/pcp/pcf032
|
| PMID |
11917081
|
| MeSH |
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Acetyltransferases / genetics
Arabidopsis / enzymology
Arabidopsis / genetics*
Carbazoles / pharmacology
Cell Line
Cells, Cultured
Dihydrolipoamide Dehydrogenase / genetics
Dihydrolipoyllysine-Residue Acetyltransferase
Dimethyl Sulfoxide / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Indole Alkaloids
Ketone Oxidoreductases / genetics*
Luciferases / genetics
Luciferases / metabolism
Molecular Sequence Data
Multienzyme Complexes / genetics*
Okadaic Acid / pharmacology
Plants, Genetically Modified
Promoter Regions, Genetic / genetics*
Protein Kinase C / antagonists & inhibitors
Pyruvate Dehydrogenase Complex / genetics
Sucrose / pharmacology*
Tobacco / cytology
|
| IF |
4.062
|
| 引用数 |
21
|
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |
