RRC ID 20095
Author Sato Y, Murakami M, Miyazawa K, Hori K.
Title Purification and characterization of a novel lectin from a freshwater cyanobacterium, Oscillatoria agardhii.
Journal Comp. Biochem. Physiol. B, Biochem. Mol. Biol.
Abstract In the survey of 14 species of laboratory-cultured cyanobacteria for hemagglutinins, we newly detected the activity in two species, Oscillatoria agardhii, strain NIES-204, and Phormidium foveolarum, strain NIES-503. From the extract of O. agardhii, which showed the highest activity with trypsin-treated erythrocytes of rabbit, a lectin was purified to homogeneity by the combination of precipitation with (NH4)2SO4, gel filtration, hydrophobic chromatography and reverse phase chromatography. The purified lectin, designated OAA, was a monomeric protein with an apparent molecular weight of 13,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 16,000 on gel filtration. The amino acid composition was rich in glycine and acidic amino acids. The hemagglutination activity was inhibited by glycoproteins such as yeast mannan, but not by any of the monosaccharides tested. The activity was stable over a wide range of pH (4-11) and at a high temperature of 80 degrees C, and independent on the presence of divalent cations. The features of OAA resembled those of many of lectins from marine macroalgae. The sequence of amino-terminal residues of OAA was determined as ALYNVENQWGGSSAPWNEGG, which was highly homologous to those of lectins from macroalgae of the genus Eucheuma and that of a myxobacterium Myxococcus xanthus hemagglutinin.
Volume 125(2)
Pages 169-77
Published 2000-2
PII S0305049199001649
PMID 10817903
MeSH Amino Acid Motifs Amino Acid Sequence Animals Bacteria / chemistry Bacterial Proteins* Carbohydrate Metabolism Carrier Proteins / chemistry* Carrier Proteins / metabolism Cell Extracts Chromatography, Gel Cyanobacteria / chemistry* Cyanobacteria / genetics Electrophoresis, Polyacrylamide Gel Enzyme Stability Glycoproteins / metabolism Hemagglutination Inhibition Tests Hemagglutinins / chemistry* Hemagglutinins / isolation & purification* Hemagglutinins / metabolism Hemagglutinins / pharmacology Humans Lectins / chemistry* Lectins / isolation & purification* Lectins / metabolism Lectins / pharmacology Molecular Sequence Data Protein Conformation Rabbits Rhodophyta / chemistry
IF 1.684
Times Cited 26
WOS Category ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Algae NIES-204