RRC ID 2017
Author Takahashi M, Kurosaka K, Kobayashi Y.
Title Immature dendritic cells reduce proinflammatory cytokine production by a coculture of macrophages and apoptotic cells in a cell-to-cell contact-dependent manner.
Journal J Leukoc Biol
Abstract We have demonstrated that phagocytosis of late apoptotic cells by mouse macrophages leads to the production of proinflammatory cytokines, notably macrophage-inflammatory protein (MIP-2), and therefore, a yet-unknown mechanism(s) should keep our body free of inflammation. In this study, we examined the effect of the addition of immature dendritic cells (iDCs) to a coculture of macrophages and apoptotic cells on MIP-2 production and phagocytosis by macrophages. The addition of iDCs to the coculture reduced MIP-2 production significantly but unexpectedly enhanced the phagocytosis by macrophages. Further study revealed that the reduction of MIP-2 production was dependent on cell-to-cell contact partly involving the beta(2) integrin family Mac-1. In addition, anti-inflammatory cytokines, interleukin-10 and transforming growth factor-beta, were involved in the reduction of MIP-2 production, as antibodies against these cytokines recovered MIP-2 production. Both cytokines were expressed by iDCs more significantly than macrophages at the mRNA levels, although they were hardly detected in the supernatant at the protein levels, suggesting that minute amounts of these anti-inflammatory cytokines were produced mainly by iDCs to block MIP-2 production in a cell-to-cell contact-dependent manner. Thus, this study reveals a new mechanism by which MIP-2 production by macrophages phagocytosing apoptotic cells is prevented.
Volume 75(5)
Pages 865-73
Published 2004-5-1
DOI 10.1189/jlb.1003471
PII jlb.1003471
PMID 14761936
MeSH Animals Apoptosis / immunology* Cell Communication / immunology* Cells, Cultured Chemokine CXCL2 Coculture Techniques Cytokines / biosynthesis* Cytokines / pharmacology Dendritic Cells / cytology Dendritic Cells / immunology* Down-Regulation / immunology Inflammation / immunology Interleukin-10 / pharmacology Interleukin-6 / biosynthesis Macrophages / cytology* Macrophages / immunology Male Mice Mice, Inbred C57BL Monokines / biosynthesis T-Lymphocytes Transforming Growth Factor beta / pharmacology
IF 3.757
Times Cited 5
DNA material pRx-mGMCSF-bsr (RDB01888)