| RRC ID |
204
|
| 著者 |
Uehara M, Haramoto Y, Sekizaki H, Takahashi S, Asashima M.
|
| タイトル |
Chromosome mapping of Xenopus tropicalis using the G- and Ag-bands: tandem duplication and polyploidization of larvae heads.
|
| ジャーナル |
Dev Growth Differ
|
| Abstract |
Developmental cytogenetic analyses of Xenopus tropicalis larvae from two origins were performed on stage 27-34 heads treated with colchicine. Standard G-band karyotyping using trypsin and chromosome mapping of 184 bands were examined. Although the main karyotype was 2n = 20, polyploidy (3n = 30 or 4n = 40) and aneuploidy were detected in each individual treated with colchicine, even those treated for only 1 h. The percentage of polyploid karyotypes was 10-20% across the total of measured metaphases. The mean mitotic index was 0.10. Chromosomal breaks and exchanges were detected at the secondary constriction of chromosomes 5 or 6. Ag-band detection showed clearly positive staining at the secondary constriction of chromosome 5, which corresponds to the nucleolar organizer region. Tandem duplication of negative G-bands at the secondary constriction of chromosome 6 and the short arm of chromosome 10 was suggested by this study. X. tropicalis thus provides a good model to study the mechanism and effects of chromosomal abnormalities, gene mapping and tissue specific gene expression in the developmental process.
|
| 巻・号 |
44(5)
|
| ページ |
427-36
|
| 公開日 |
2002-10-1
|
| DOI |
10.1046/j.1440-169x.2002.00656.x
|
| PII |
656
|
| PMID |
12392576
|
| MeSH |
Animals
Chromosome Banding*
Chromosome Mapping*
Female
Gene Duplication*
Head
Karyotyping
Larva / genetics
Male
Polyploidy
Xenopus / genetics*
|
| IF |
1.723
|
| 引用数 |
9
|
|
WOS 分野
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| ツメガエル・イモリ |
|
