RRC ID 2050
Author Hirai S, Kawaguchi A, Hirasawa R, Baba M, Ohnishi T, Ohno S.
Title MAPK-upstream protein kinase (MUK) regulates the radial migration of immature neurons in telencephalon of mouse embryo.
Journal Development
Abstract The radial migration of differentiating neurons provides an essential step in the generation of laminated neocortex, although its molecular mechanism is not fully understood. We show that the protein levels of a JNK activator kinase, MUK/DLK/ZPK, and JNK activity increase potently and temporally in newly generated neurons in developing mouse telencephalon during radial migration. The ectopic expression of MUK/DLK/ZPK in neural precursor cells in utero impairs radial migration, whereas it allows these cells to leave the ventricular zone and differentiate into neural cells. The MUK/DLK/ZPK protein is associated with dotted structures that are frequently located along microtubules and with Golgi apparatus in cultured embryonic cortical cells. In COS-1 cells, MUK/DLK/ZPK overexpression impairs the radial organization of microtubules without massive depolymerization. These results suggest that MUK/DLK/ZPK and JNK regulate radial cell migration via microtubule-based events.
Volume 129(19)
Pages 4483-95
Published 2002-10-1
DOI 10.1242/dev.129.19.4483
PMID 12223406
MeSH 3T3 Cells Animals COS Cells Cell Line, Transformed Cell Movement / physiology* Chlorocebus aethiops Gene Expression Humans MAP Kinase Kinase Kinases / genetics MAP Kinase Kinase Kinases / metabolism* Mice Mice, Inbred ICR Microtubules / physiology Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinase 9 Mitogen-Activated Protein Kinases / metabolism* Neocortex / embryology Neurons / cytology Neurons / physiology* Protein Serine-Threonine Kinases* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction Stem Cells / cytology Stem Cells / physiology* Telencephalon / cytology*
IF 5.611
Times Cited 63
DNA material AxCALacZ (RDB1745)