Reference - Detail
| RRC ID | 20690 |
|---|---|
| Author | Ohmori M, Hasunuma K, Furukawa K. |
| Title | GTP-binding proteins in a cyanobacterium Anabaena cylindrica. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
GTP-binding proteins were detected in a crude extract containing membrane components of Anabaena cylindrica. The crude extract was treated with 1% Lubrol PX and was fractionated by gel filtration. The binding of [35S]GTP gamma S to GTP-binding proteins was prevented in the presence of 0.1 mM GTP and in the presence of 0.1 mM ATP. Six fractions of these GTP-binding proteins, tentatively designated GA1 to GA6, were ADP-ribosylated by pertussis toxin. GA3, GA4 and GA5 had Km values of 10, 60 and 7 nM, respectively. The molecular weights of some of these GTP-binding proteins were reduced after being labelled with [35S]GTP gamma S. |
| Volume | 151(1) |
| Pages | 215-21 |
| Published | 1988-2-29 |
| DOI | 10.1016/0006-291x(88)90581-5 |
| PII | 0006-291X(88)90581-5 |
| PMID | 3126734 |
| MeSH | Binding, Competitive Cell Membrane / analysis Chromatography, Gel Cyanobacteria / analysis* GTP-Binding Proteins / analysis* GTP-Binding Proteins / metabolism Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate / analogs & derivatives Guanosine Triphosphate / metabolism Thionucleotides / metabolism |
| IF | 2.985 |
| Times Cited | 7 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Algae | NIES-19 |