RRC ID 20748
Author Toda H, Yazawa M, Yagi K.
Title Amino acid sequence of calmodulin from Euglena gracilis.
Journal Eur J Biochem
Abstract The complete amino acid sequence of calmodulin from Euglena gracilis was determined by isolation and sequence analyses of peptides derived from calmodulin by digestion with trypsin and Staphylococcus aureus V8 protease. Euglena calmodulin consists of 148 amino acid residues; it lacks tryptophan and cysteine and contains one tyrosine, three histidine and two NE-trimethyllysine residues/molecule of the protein. Its N-terminus was blocked with an acetyl group and C-terminal lysine was trimethylated. Euglena calmodulin is the first calmodulin so far examined in which the C-terminal lysine is trimethylated. The comparison of amino acid sequences between Euglena and human brain calmodulins indicated 17 amino acid substitutions in Euglena calmodulin.
Volume 205(2)
Pages 653-60
Published 1992-4-15
DOI 10.1111/j.1432-1033.1992.tb16824.x
PMID 1572365
MeSH Amino Acid Sequence Animals Calmodulin / chemistry* Calmodulin / genetics Calmodulin / isolation & purification Chromatography, High Pressure Liquid Euglena gracilis / genetics Euglena gracilis / metabolism* Humans Molecular Sequence Data Peptide Fragments / isolation & purification Sequence Homology, Nucleic Acid Serine Endopeptidases Trypsin
Times Cited 16
Algae NIES-48