RRC ID 20789
Author Yoshioka S, Taniguchi F, Miura K, Inoue T, Yamano T, Fukuzawa H.
Title The novel Myb transcription factor LCR1 regulates the CO2-responsive gene Cah1, encoding a periplasmic carbonic anhydrase in Chlamydomonas reinhardtii.
Journal Plant Cell
Abstract Chlamydomonas reinhardtii acclimates to CO2-limiting stress by inducing a set of genes for a carbon-concentrating mechanism (CCM). This set includes the gene Cah1, which encodes a periplasmic carbonic anhydrase. Although physiological aspects of CO2response have been extensively studied, regulatory components, such as transcription factors involved in the acclimation, have not been well described in eukaryotic microalgae. Using an arylsulfatase gene driven by the Cah1 promoter, a regulatory mutant of Cah1 was isolated and named lcr1 (for low-CO2 stress response). The photosynthetic affinity for inorganic carbon of lcr1 was reduced compared with that of wild-type cells. Expression of three low-CO2-inducible genes, Cah1, Lci1, and Lci6, were regulated by LCR1 as shown by cDNA array and RNA gel blot analyses. The Lcr1 gene encodes a protein of 602 amino acids containing a single Myb domain, which binds to the Cah1-promoter region. Expression of Lcr1 was induced by lowering CO2 levels and controlled by the regulatory factor CCM1. These results suggest that LCR1 transmits the low CO2 signal to at least three CO2-responsive genes and then fully induces CCM.
Volume 16(6)
Pages 1466-77
Published 2004-6-1
DOI 10.1105/tpc.021162
PII tpc.021162
PMID 15155888
PMC PMC490039
MeSH Adaptor Proteins, Signal Transducing Algal Proteins / genetics Algal Proteins / metabolism Amino Acid Sequence Animals Carbon Dioxide / pharmacology* Carbonic Anhydrases / genetics* Carbonic Anhydrases / metabolism Chlamydomonas reinhardtii / drug effects Chlamydomonas reinhardtii / enzymology Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / metabolism DNA / genetics DNA / metabolism Gene Expression Regulation, Enzymologic / drug effects* Genetic Complementation Test Molecular Sequence Data Mutation / genetics Oligonucleotide Array Sequence Analysis Periplasm / enzymology* Plant Proteins Promoter Regions, Genetic / genetics Protein Structure, Tertiary Proto-Oncogene Proteins c-myb / chemistry Proto-Oncogene Proteins c-myb / genetics Proto-Oncogene Proteins c-myb / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Substrate Specificity Trans-Activators / genetics Trans-Activators / metabolism Transcription Factors / chemistry Transcription Factors / metabolism*
IF 9.618
Times Cited 75
Algae NIES-2235