RRC ID 20793
Author Kubo T, Saito T, Fukuzawa H, Matsuda Y.
Title Two tandemly-located matrix metalloprotease genes with different expression patterns in the chlamydomonas sexual cell cycle.
Journal Curr. Genet.
Abstract Cloning of the gametolysin gene, mmp1, of Chlamydomonas reinhardtii revealed that the gene has six introns in its coding region and that a novel structurally related gene. mmp2, is located just upstream of the mmp1 gene. The mmp2 gene has ten introns and the deduced amino acid sequence has the hallmark motif characteristics of metzincin-type matrix metalloproteases. Southern analysis showed that mmp1 and mmp2 are single copy genes and that a RFLP is found between the mt+ and mt- strains employed. Northern analysis revealed that the two genes are expressed at different stages in the Chlamydomonas sexual cell cycle: steady-state levels of mmp1 mRNA increased during gametogenesis, while those of mmp2 mRNA increased in young zygotes. Transcription of mmp2 was insensitive to cycloheximide and did not occur when a non-fusing mutant, fus mt+, was mated with wild-type mt- gametes, suggesting that mmp2 is a member of the early zygote-specific genes, which are under the regulation of transcription factors pre-existing in gametes and activated by cytoplasmic fusion.
Volume 40(2)
Pages 136-43
Published 2001-9
PMID 11680823
MeSH Amino Acid Sequence Animals Chlamydomonas reinhardtii / drug effects Chlamydomonas reinhardtii / physiology* Cloning, Molecular Cycloheximide / pharmacology Gene Expression Regulation Gene Order Matrix Metalloproteinase 1 / genetics* Matrix Metalloproteinase 1 / metabolism Matrix Metalloproteinase 2 / drug effects Matrix Metalloproteinase 2 / genetics* Matrix Metalloproteinase 2 / metabolism Molecular Sequence Data Mutation Reproduction / genetics Sequence Analysis Sequence Homology, Amino Acid Zygote
IF 3.464
Times Cited 21
Algae NIES-2235