| RRC ID |
20794
|
| Author |
Asamizu E, Miura K, Kucho K, Inoue Y, Fukuzawa H, Ohyama K, Nakamura Y, Tabata S.
|
| Title |
Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii.
|
| Journal |
DNA Res
|
| Abstract |
To characterize genes whose expression is induced in carbon-stress conditions, 12,969 and 13,450 5'-end expressed sequence tags (ESTs) were generated from cells grown in low-CO2 and high-CO2 conditions of the unicellular green alga, Chlamydomonas reinhardtii. These ESTs were clustered into 4436 and 3566 non-redundant EST groups, respectively. Comparison of their sequences with those of 3433 non-redundant ESTs previously generated from the cells under the standard growth condition indicated that 2665 and 1879 EST groups occurred only in the low-CO2 and high-CO2 populations, respectively. It was also noted that 96.2% and 96.0% of the cDNA species respectively obtained from the low-CO2 and high-CO2 conditions had no similar EST sequence deposited in the public databases. The EST species identified only in the low-CO2 treated cells included genes previously reported to be expressed specifically in low-CO2 acclimatized cells, suggesting that the ESTs generated in this study will be a useful source for analysis of genes related to carbon-stress acclimatization. The sequence information and search results of each clone will appear at the web site: http://www.kazusa.or.jp/en/plant/chlamy/EST/.
|
| Volume |
7(5)
|
| Pages |
305-7
|
| Published |
2000-10-31
|
| DOI |
10.1093/dnares/7.5.305
|
| PMID |
11089912
|
| MeSH |
Animals
Carbon Dioxide / pharmacology
Chlamydomonas reinhardtii / drug effects
Chlamydomonas reinhardtii / genetics*
Chlamydomonas reinhardtii / physiology
DNA, Complementary / genetics
Expressed Sequence Tags*
Gene Library
|
| IF |
4.009
|
| Times Cited |
103
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Algae |
NIES-2235 |
