論文 - 詳細
| RRC ID | 20804 |
|---|---|
| 著者 | Ha JH, Hidaka T, Tsuno H. |
| タイトル | Quantification of toxic Microcystis and evaluation of its dominance ratio in blooms using real-time PCR. |
| ジャーナル | Environ Sci Technol |
| Abstract |
Microcystins are produced by cyanobacteria carrying microcystin synthetase (mcy) genes in blooms. The present study investigated seasonal variations in concentrations of mcy A and 16S rRNA genes, encoded in the genus Microcystis using real-time PCR, and measured the water quality variables and microcystin concentrations in a hypereutrophic fish pond between June and November 2007. Prior to field survey, the relationship between axenic Microcystis aeruginosa NIES 102 cell abundance and concentrations of mcy A and 16S rRNA genes was determined by real-time PCR in the laboratory. Based on the quantitative relationship, microcystin-producing and total Microcystis cell numbers in the study site were estimated. The average dominance ratio of microcystin-producing strains in the total Microcystis population was approximately 80% and microcystin concentration was highly associated with toxic Microcystis cell numbers equivalent of mcy A gene. It was also observed that total nitrogen concentration was associated with development of Microcystis blooms, and high concentrations of NOx and NH4 increased microcystin production by promoting growth of microcystin-producing Microcystis strains. |
| 巻・号 | 43(3) |
| ページ | 812-8 |
| 公開日 | 2009-2-1 |
| DOI | 10.1021/es801265f |
| PMID | 19245020 |
| MeSH | Base Sequence DNA Primers Microcystins / analysis Microcystins / toxicity Microcystis / genetics Microcystis / growth & development* Microcystis / metabolism Polymerase Chain Reaction |
| IF | 7.864 |
| 引用数 | 62 |
| WOS 分野 | ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-102 |