Reference - Detail
| RRC ID | 20804 |
|---|---|
| Author | Ha JH, Hidaka T, Tsuno H. |
| Title | Quantification of toxic Microcystis and evaluation of its dominance ratio in blooms using real-time PCR. |
| Journal | Environ Sci Technol |
| Abstract |
Microcystins are produced by cyanobacteria carrying microcystin synthetase (mcy) genes in blooms. The present study investigated seasonal variations in concentrations of mcy A and 16S rRNA genes, encoded in the genus Microcystis using real-time PCR, and measured the water quality variables and microcystin concentrations in a hypereutrophic fish pond between June and November 2007. Prior to field survey, the relationship between axenic Microcystis aeruginosa NIES 102 cell abundance and concentrations of mcy A and 16S rRNA genes was determined by real-time PCR in the laboratory. Based on the quantitative relationship, microcystin-producing and total Microcystis cell numbers in the study site were estimated. The average dominance ratio of microcystin-producing strains in the total Microcystis population was approximately 80% and microcystin concentration was highly associated with toxic Microcystis cell numbers equivalent of mcy A gene. It was also observed that total nitrogen concentration was associated with development of Microcystis blooms, and high concentrations of NOx and NH4 increased microcystin production by promoting growth of microcystin-producing Microcystis strains. |
| Volume | 43(3) |
| Pages | 812-8 |
| Published | 2009-2-1 |
| DOI | 10.1021/es801265f |
| PMID | 19245020 |
| MeSH | Base Sequence DNA Primers Microcystins / analysis Microcystins / toxicity Microcystis / genetics Microcystis / growth & development* Microcystis / metabolism Polymerase Chain Reaction |
| IF | 7.864 |
| Times Cited | 62 |
| WOS Category | ENVIRONMENTAL SCIENCES ENGINEERING, ENVIRONMENTAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-102 |