RRC ID |
20804
|
著者 |
Ha JH, Hidaka T, Tsuno H.
|
タイトル |
Quantification of toxic Microcystis and evaluation of its dominance ratio in blooms using real-time PCR.
|
ジャーナル |
Environ Sci Technol
|
Abstract |
Microcystins are produced by cyanobacteria carrying microcystin synthetase (mcy) genes in blooms. The present study investigated seasonal variations in concentrations of mcy A and 16S rRNA genes, encoded in the genus Microcystis using real-time PCR, and measured the water quality variables and microcystin concentrations in a hypereutrophic fish pond between June and November 2007. Prior to field survey, the relationship between axenic Microcystis aeruginosa NIES 102 cell abundance and concentrations of mcy A and 16S rRNA genes was determined by real-time PCR in the laboratory. Based on the quantitative relationship, microcystin-producing and total Microcystis cell numbers in the study site were estimated. The average dominance ratio of microcystin-producing strains in the total Microcystis population was approximately 80% and microcystin concentration was highly associated with toxic Microcystis cell numbers equivalent of mcy A gene. It was also observed that total nitrogen concentration was associated with development of Microcystis blooms, and high concentrations of NOx and NH4 increased microcystin production by promoting growth of microcystin-producing Microcystis strains.
|
巻・号 |
43(3)
|
ページ |
812-8
|
公開日 |
2009-2-1
|
DOI |
10.1021/es801265f
|
PMID |
19245020
|
MeSH |
Base Sequence
DNA Primers
Microcystins / analysis
Microcystins / toxicity
Microcystis / genetics
Microcystis / growth & development*
Microcystis / metabolism
Polymerase Chain Reaction
|
IF |
7.864
|
引用数 |
62
|
WOS 分野
|
ENVIRONMENTAL SCIENCES
ENGINEERING, ENVIRONMENTAL
|
リソース情報 |
藻類 |
NIES-102 |